BACKGROUND: In this study, we aimed to determine the relation of TLR-4 Asp299Gly and Thr399Ile polymorphisms and monocyte/neutrophil TLR-4 expression to febrile urinary tract infection (UTI) and renal scar development in children. METHODS: The study was performed in children with a history of febrile UTI. Patients with and without renal scarring were classified as group 1 and group 2, respectively, while the control cases in our previous study were used as the control group (group 3). All three groups were compared for the rate of TLR-4 Asp299Gly and Thr399Ile polymorphisms, and for basal and lipopolysaccharide-stimulated monocyte/neutrophil TLR-4 expression levels. RESULTS: There were 168 patients (86 in group 1, 82 in group 2) and 120 control cases. Monocyte/neutrophil TLR-4 expression levels were similar in groups 1 and 2. However, both groups had lower TLR-4 expression than group 3. The rate of TLR-4 Asp299Gly polymorphism was not different in all groups. TLR-4 Thr399Ile polymorphism was higher in groups 1 and 2 than in group 3 (14.0, 12.2, and 2.0 %, respectively), while group 1 and group 2 were not different. Furthermore, monocyte TLR-4 expression level was lower in those having TLR-4 Thr399Ile polymorphism than in those without this polymorphism. CONCLUSIONS: Patients with febrile UTI had more frequent TLR-4 Thr399Ile polymorphism and lower monocyte/neutrophil TLR-4 expression. These findings indicate that children carrying TLR-4 Thr399Ile polymorphism and/or having low level of monocyte/neutrophil TLR-4 expression have a tendency to develop febrile UTI. However, we could not show the association of TLR-4 polymorphisms and of TLR-4 expression level to renal scarring.
BACKGROUND: In this study, we aimed to determine the relation of TLR-4 Asp299Gly and Thr399Ile polymorphisms and monocyte/neutrophil TLR-4 expression to febrile urinary tract infection (UTI) and renal scar development in children. METHODS: The study was performed in children with a history of febrile UTI. Patients with and without renal scarring were classified as group 1 and group 2, respectively, while the control cases in our previous study were used as the control group (group 3). All three groups were compared for the rate of TLR-4 Asp299Gly and Thr399Ile polymorphisms, and for basal and lipopolysaccharide-stimulated monocyte/neutrophil TLR-4 expression levels. RESULTS: There were 168 patients (86 in group 1, 82 in group 2) and 120 control cases. Monocyte/neutrophil TLR-4 expression levels were similar in groups 1 and 2. However, both groups had lower TLR-4 expression than group 3. The rate of TLR-4 Asp299Gly polymorphism was not different in all groups. TLR-4 Thr399Ile polymorphism was higher in groups 1 and 2 than in group 3 (14.0, 12.2, and 2.0 %, respectively), while group 1 and group 2 were not different. Furthermore, monocyte TLR-4 expression level was lower in those having TLR-4 Thr399Ile polymorphism than in those without this polymorphism. CONCLUSIONS:Patients with febrile UTI had more frequent TLR-4 Thr399Ile polymorphism and lower monocyte/neutrophil TLR-4 expression. These findings indicate that children carrying TLR-4 Thr399Ile polymorphism and/or having low level of monocyte/neutrophil TLR-4 expression have a tendency to develop febrile UTI. However, we could not show the association of TLR-4 polymorphisms and of TLR-4 expression level to renal scarring.
Authors: M R Ditchfield; J F de Campo; T M Nolan; D J Cook; K Grimwood; H R Powell; R Sloane; S Cahill Journal: AJR Am J Roentgenol Date: 1994-06 Impact factor: 3.959
Authors: Bart Ferwerda; Matthew B B McCall; Santos Alonso; Evangelos J Giamarellos-Bourboulis; Maria Mouktaroudi; Neskuts Izagirre; Din Syafruddin; Gibson Kibiki; Tudor Cristea; Anneke Hijmans; Lutz Hamann; Shoshana Israel; Gehad ElGhazali; Marita Troye-Blomberg; Oliver Kumpf; Boubacar Maiga; Amagana Dolo; Ogobara Doumbo; Cornelus C Hermsen; Anton F H Stalenhoef; Reinout van Crevel; Han G Brunner; Djin-Ye Oh; Ralf R Schumann; Concepcion de la Rúa; Robert Sauerwein; Bart-Jan Kullberg; André J A M van der Ven; Jos W M van der Meer; Mihai G Netea Journal: Proc Natl Acad Sci U S A Date: 2007-10-09 Impact factor: 11.205
Authors: Mark C Dessing; Jesper Kers; Jeffrey Damman; Henri G D Leuvenink; Harry van Goor; Jan-Luuk Hillebrands; Bouke G Hepkema; Harold Snieder; Jacob van den Born; Martin H de Borst; Stephan J L Bakker; Gerjan J Navis; Rutger J Ploeg; Sandrine Florquin; Marc Seelen; Jaklien C Leemans Journal: PLoS One Date: 2015-10-07 Impact factor: 3.240