| Literature DB >> 23606254 |
Ana Lopez-Campistrous1, Carlos Fernandez-Patron.
Abstract
We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/Western and sucrose gradient ultracentrifugation/SDS-PAGE/mass spectrometry are optimized to characterize the brain mitochondrial proteome. This approach allows for a comprehensive identification of mitochondrial proteomic differences between health and disease conditions.Entities:
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Year: 2013 PMID： 23606254 DOI： 10.1007/978-1-62703-386-2_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745