Biotransformation of T-2 toxin and diacetoxyscirpenol in the isolated perfused rat liver.

CONCLUSION: Twelve metabolites of T-2 toxin and two metabolites of DAS have been detected by GLC-MS in the perfusate of isolated rat livers. Less than 50% of the administered dose was recovered in the perfusate. However, preliminary results of the analysis of bile show that considerable amounts of T-2 toxin and DAS were excreted mainly as glucuronide conjugates. The concurrence of the data reported here with the results of in vivo studies indicate that the isolaged perfused rat liver system is suitable in studying the metabolism of trichothecence mycotoxins.