Comparison of immunoaffinity columns with Florisil/C18 columns for the determination of aflatoxins in animal feed and maize.

A study of the performance of the EC method and an Immunoaffinity method for aflatoxins B1,B2,G1 and G2 in animal feed and maize was performed. The extraction and HPLC analysis of both procedures were comparable,hence a direct comparison of the performance of the alternative clean- up columns was made. Throughout the study the results were similar for both methods,however,the main advantage of the Immunoaffinity method was fewer manipulations were required. Therefore the method was easier to use,less solvent was required and greater sample throughput (approximately 2 fold) was obtained.