UNLABELLED: To our knowledge, this is the first study to examine the relationship between guanine nucleotide binding protein β-1 (GNB1) mRNA expression and clinicopathological parameters. Furthermore, the correlations between GNB1, Rictor and the mammalian target of rapamycin (mTOR) were also investigated. MATERIALS AND METHODS: Breast cancer tissues (n=136) and normal tissues (n=31) underwent reverse transcription and quantitative polymerase chain reaction. Transcript levels were correlated with clinicopathological data. RESULTS: Higher mRNA transcript levels of GNB1 were found in the breast cancer specimens in paired samples (p=0.0029). The mRNA expression of GNB1 increased with TNM stage (TNM1 vs. TNM2/3/4, p=0.036), tumour grade (grade 2 vs. 3, p=0.006), in ductal tumours (p=0.0081), and was associated with adverse patient outcomes (mortality vs. disease-free survival: 4.9 vs. 0.01, p=0.027). GNB1 was positively-correlated with mTOR (r=0.525, p<0.000001) and Rictor (r=0.388, p=0.0000606). CONCLUSION: These observations may suggest that GNB1 plays an important role in the mTOR-related anti-apoptosis pathway and can potentially be targeted in the treatment of human breast cancer.
UNLABELLED: To our knowledge, this is the first study to examine the relationship between guanine nucleotide binding protein β-1 (GNB1) mRNA expression and clinicopathological parameters. Furthermore, the correlations between GNB1, Rictor and the mammalian target of rapamycin (mTOR) were also investigated. MATERIALS AND METHODS:Breast cancer tissues (n=136) and normal tissues (n=31) underwent reverse transcription and quantitative polymerase chain reaction. Transcript levels were correlated with clinicopathological data. RESULTS: Higher mRNA transcript levels of GNB1 were found in the breast cancer specimens in paired samples (p=0.0029). The mRNA expression of GNB1 increased with TNM stage (TNM1 vs. TNM2/3/4, p=0.036), tumour grade (grade 2 vs. 3, p=0.006), in ductal tumours (p=0.0081), and was associated with adverse patient outcomes (mortality vs. disease-free survival: 4.9 vs. 0.01, p=0.027). GNB1 was positively-correlated with mTOR (r=0.525, p<0.000001) and Rictor (r=0.388, p=0.0000606). CONCLUSION: These observations may suggest that GNB1 plays an important role in the mTOR-related anti-apoptosis pathway and can potentially be targeted in the treatment of humanbreast cancer.
Authors: William Putzbach; Quan Q Gao; Monal Patel; Stijn van Dongen; Ashley Haluck-Kangas; Aishe A Sarshad; Elizabeth T Bartom; Kwang-Youn A Kim; Denise M Scholtens; Markus Hafner; Jonathan C Zhao; Andrea E Murmann; Marcus E Peter Journal: Elife Date: 2017-10-24 Impact factor: 8.140
Authors: Brooke L Fridley; Sebastian M Armasu; Mine S Cicek; Melissa C Larson; Chen Wang; Stacey J Winham; Kimberly R Kalli; Devin C Koestler; David N Rider; Viji Shridhar; Janet E Olson; Julie M Cunningham; Ellen L Goode Journal: BMC Med Genomics Date: 2014-04-28 Impact factor: 3.063