Olivier Perche1, Juliette Vergnaud-Gauduchon1, Christine Morand2, Claude Dubray3, Andrzej Mazur2, Marie-Paule Vasson4. 1. Clermont Université, Université d'Auvergne, UMR 1019, Unité de Nutrition Humaine, CRNH-Auvergne, CLARA, BP 10448, F-63000 Clermont-Ferrand, France. 2. Centre de recherche INRA, UMR 1019 INRA Université d'Auvergne, Unité de Nutrition Humaine, Clermont-Ferrand Theix, France. 3. CHU Clermont-Ferrand, Centre de Pharmacologie Clinique, Inserm, CIC 501, Univ Clermont 1, Faculté de Médecine, F-63001 Clermont-Ferrand, France. 4. Centre anticancéreux Jean-Perrin, CHU, Unité de Nutrition, CLARA, F-63000 Clermont-Ferrand, France. Electronic address: m-paule.vasson@udamail.fr.
Abstract
BACKGROUND & AIMS:Polyphenols exert a variety of biological properties, including antioxidant, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men, we investigated the effects of orange juice and its major polyphenolic compound hesperidin on a panel of immune cell functions, including cytokine secretion by leukocytes, lytic activity of NK cells, and the Reactive Oxygen Species (ROS) burst by polymorphonuclear neutrophil cells (PMN). METHODS: The protocol design was divided into three 4-week treatment periods separated by 3-week wash-out intervals, for total study duration of 18 weeks. During treatment periods, volunteers (n = 24) consumed daily 500 mL of orange juice, or an isocaloric control beverage with hesperidin (292 mg in a capsule), or of the same control beverage with a placebo. RESULTS: Whatever the intake was, no variations were recorded on leukocyte subset distributions (PMN, B and T lymphocytes, NK cells and monocytes), ROS production by stimulated PMNs, lytic activity of NK cells or cytokine production capacity of leukocytes in well-nourished healthy volunteers. CONCLUSIONS: We show that consumption within the usual daily intake range of orange juice and its major polyphenol hesperidin do not induce immunomodulation of cell immune function in healthy well-nourished humans.
RCT Entities:
BACKGROUND & AIMS:Polyphenols exert a variety of biological properties, including antioxidant, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men, we investigated the effects of orange juice and its major polyphenolic compound hesperidin on a panel of immune cell functions, including cytokine secretion by leukocytes, lytic activity of NK cells, and the Reactive Oxygen Species (ROS) burst by polymorphonuclear neutrophil cells (PMN). METHODS: The protocol design was divided into three 4-week treatment periods separated by 3-week wash-out intervals, for total study duration of 18 weeks. During treatment periods, volunteers (n = 24) consumed daily 500 mL of orange juice, or an isocaloric control beverage with hesperidin (292 mg in a capsule), or of the same control beverage with a placebo. RESULTS: Whatever the intake was, no variations were recorded on leukocyte subset distributions (PMN, B and T lymphocytes, NK cells and monocytes), ROS production by stimulated PMNs, lytic activity of NK cells or cytokine production capacity of leukocytes in well-nourished healthy volunteers. CONCLUSIONS: We show that consumption within the usual daily intake range of orange juice and its major polyphenol hesperidin do not induce immunomodulation of cell immune function in healthy well-nourished humans.
Authors: Mariana S Dorna; Elizabete M S Barbosa; Matheus A Callegari; Suzana E Tanni; Fernanda Chiuso-Minicucci; Tainara F Felix; Ana L Seneda; Camila R Correa; Ana A H Fernandes; Paula S Azevedo; Bertha F Polegato; Marcelo M Rogero; Sergio A R Paiva; Leonardo A M Zornoff; Patricia P Reis; Marcos F Minicucci Journal: Front Nutr Date: 2021-12-24