OBJECTIVES: The aim of this study was to evaluate the anti-inflammatory effect of a sulphated polysaccharide fraction (PLS) extracted from the alga Hypnea musciformis and investigate the possible involvement of the nitric oxide (NO) pathway in this effect. METHODS: The anti-inflammatory activity of PLS was evaluated using inflammatory agents (carrageenan and dextran) to induce paw oedema and peritonitis in Swiss mice. Samples of paw tissue and peritoneal fluid were removed to determine myeloperoxidase (MPO) activity, NO3 /NO2 levels, and interleukin-1β (IL-1β) level. The involvement of NO in the modulation of neutrophil migration in carrageenan-induced paw oedema or peritonitis was also investigated. KEY FINDINGS: Compared with vehicle-treated mice, mice pretreated with PLS (10 mg/kg) inhibited carrageenan-induced and dextran-induced oedema; it also inhibited total and differential peritoneal leucocyte counts in a model of peritonitis. These PLS effects were reversed by l-arginine treatment and recovered with the administration of a NO synthase blocker (aminoguanidine). Furthermore, PLS reduced the MPO activity, decreased IL-1β levels, and increased NO3 /NO2 levels in the peritoneal cavity. CONCLUSIONS: PLS reduced the inflammatory response by modulating neutrophil migration, which appeared to be dependent on the NO pathway.
OBJECTIVES: The aim of this study was to evaluate the anti-inflammatory effect of a sulphated polysaccharide fraction (PLS) extracted from the alga Hypnea musciformis and investigate the possible involvement of the nitric oxide (NO) pathway in this effect. METHODS: The anti-inflammatory activity of PLS was evaluated using inflammatory agents (carrageenan and dextran) to induce paw oedema and peritonitis in Swiss mice. Samples of paw tissue and peritoneal fluid were removed to determine myeloperoxidase (MPO) activity, NO3 /NO2 levels, and interleukin-1β (IL-1β) level. The involvement of NO in the modulation of neutrophil migration in carrageenan-induced paw oedema or peritonitis was also investigated. KEY FINDINGS: Compared with vehicle-treated mice, mice pretreated with PLS (10 mg/kg) inhibited carrageenan-induced and dextran-induced oedema; it also inhibited total and differential peritoneal leucocyte counts in a model of peritonitis. These PLS effects were reversed by l-arginine treatment and recovered with the administration of a NO synthase blocker (aminoguanidine). Furthermore, PLS reduced the MPO activity, decreased IL-1β levels, and increased NO3 /NO2 levels in the peritoneal cavity. CONCLUSIONS:PLS reduced the inflammatory response by modulating neutrophil migration, which appeared to be dependent on the NO pathway.
Authors: Antonia Amanda Cardoso de Almeida; Renan Oliveira Silva; Lucas Antonio Duarte Nicolau; Tarcísio Vieira de Brito; Damião Pergentino de Sousa; André Luiz Dos Reis Barbosa; Rivelilson Mendes de Freitas; Luciano da Silva Lopes; Jand-Venes Rolim Medeiros; Paulo Michel Pinheiro Ferreira Journal: Inflammation Date: 2017-04 Impact factor: 4.092
Authors: Renata Fortes Santiago; Tarcisio Vieira de Brito; Jordana Maia Dias; Genilson José Dias; José Simião da Cruz; Jalles Arruda Batista; Renan Oliveira Silva; Marcellus H L P Souza; Ronaldo de Albuquerque Ribeiro; Stanley Juan Chavez Gutierrez; Rivelilson M Freitas; Jand-Venes R Medeiros; André Luiz Dos Reis Barbosa Journal: Inflammation Date: 2015-12 Impact factor: 4.092
Authors: Jordana Maia Dias; Tarcisio Vieira de Brito; Diva de Aguiar Magalhães; Pammela Weryka da Silva Santos; Jalles Arruda Batista; Eulina Gabriela do Nascimento Dias; Heliana de Barros Fernandes; Samara Rodrigues Bonfim Damasceno; Renan O Silva; Karoline S Aragão; Marcellus H L P Souza; Jand-Venes R Medeiros; André Luiz R Barbosa Journal: Inflammation Date: 2014-10 Impact factor: 4.092
Authors: Pek Xyen Tan; Krishnapriya Thiyagarasaiyar; Cheng-Yau Tan; You-Jin Jeon; Mohd Shahrul Mohd Nadzir; Yong-Jiang Wu; Liang-Ee Low; Atanas G Atanasov; Long Chiau Ming; Kai Bin Liew; Bey-Hing Goh; Yoon-Yen Yow Journal: Mar Drugs Date: 2021-05-30 Impact factor: 5.118
Authors: Adrian Wiater; Roman Paduch; Sylwia Trojnar; Adam Choma; Małgorzata Pleszczyńska; Paulina Adamczyk; Mateusz Pięt; Katarzyna Próchniak; Janusz Szczodrak; Jakub Strawa; Michał Tomczyk Journal: Plants (Basel) Date: 2020-01-14