Yang Zhao1, Bei Liu, Lei Li, Jinyi Zhong. 1. Department of Public Health, Medical Collage of Qingdao University, Qingdao 266021, China. zhaoyang5256@126.com
Abstract
OBJECTIVE: To investigate the effect of angelica keiskei chalcone (AC) on the expression of glucose transporter2 (Glut2) in liver and glucose transporter 4 (Glut4) in skeletal muscle cells of rats with type2 diabetes. METHODS: Made type2 diabetic rat models and divided into high, middle, low dose groups and diabetic control group randomly which were given chalcone 30, 10, 5 and 0 mg/kg BW respectively. Four groups were fed with the diet containing high fat. After four weeks, fasting blood glucose was determined by glucose oxidase method. Insulin was assessed by radio-immunity method. The expression levels of Glut2 in liver and Glut4 in skeletal muscle cells were analyzed by immunohistochemistry method. RESULTS: After analyzed by Image-Pro Plus on immunohistochemistry pictures, the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells in high group were (0.036 +/- 0.0030) and (0.063 +/- 0.0139), which were significantly higher than the model control group (P < 0.05). The levels of fasting blood glucose and insulin in high dose group were (12.3 +/- 1.64) mmol/L and (25.65 +/- 3.34) microIU/ml, significantly lower than those of the model control group (P < 0.05). CONCLUSION: Angelica keiskei chalcone may increase the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells, decrease fasting blood glucose and insulin of type 2 diabetic rats and improve their insulin resistance condition.
OBJECTIVE: To investigate the effect of angelica keiskeichalcone (AC) on the expression of glucose transporter2 (Glut2) in liver and glucose transporter 4 (Glut4) in skeletal muscle cells of rats with type2 diabetes. METHODS: Made type2 diabeticrat models and divided into high, middle, low dose groups and diabetic control group randomly which were given chalcone 30, 10, 5 and 0 mg/kg BW respectively. Four groups were fed with the diet containing high fat. After four weeks, fasting blood glucose was determined by glucose oxidase method. Insulin was assessed by radio-immunity method. The expression levels of Glut2 in liver and Glut4 in skeletal muscle cells were analyzed by immunohistochemistry method. RESULTS: After analyzed by Image-Pro Plus on immunohistochemistry pictures, the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells in high group were (0.036 +/- 0.0030) and (0.063 +/- 0.0139), which were significantly higher than the model control group (P < 0.05). The levels of fasting blood glucose and insulin in high dose group were (12.3 +/- 1.64) mmol/L and (25.65 +/- 3.34) microIU/ml, significantly lower than those of the model control group (P < 0.05). CONCLUSION:Angelica keiskeichalcone may increase the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells, decrease fasting blood glucose and insulin of type 2 diabeticrats and improve their insulin resistance condition.