Jeffrine Japning Rovie-Ryan1, Zainal Zahari Zainuddin, Wahap Marni, Abdul Hamid Ahmad, Laurentius N Ambu, Junaidi Payne. 1. Ex-Situ Conservation Division, Department of Wildlife and National Parks (DWNP), Peninsular Malaysia, Ministry of Natural Resources and Environment Malaysia (NRE), KM 10 Jalan Cheras, 56100 Kuala Lumpur, Malaysia ; Department of Zoology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak (UNIMAS), 94300 Kota Samarahan, Sarawak, Malaysia.
Abstract
OBJECTIVE: To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid fly. METHODS: Blood meal was recovered from the abdomen of an engorged tabanid fly (Haematopota sp.) which was captured immediately after biting a Sumatran rhino in captivity. The blood was applied on to a Whatman FTA(®) blood card. Subsequent laboratory work was conducted to extract, amplify and sequence the DNA from the sample. Validation was done by sampling the hair follicles and blood samples from the rhinoceros and subjecting it to the same laboratory process. RESULTS: BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from the rhino. CONCLUSIONS: This method could be used in the field application to noninvasively collect genetic samples. Collection of tabanids and other haematophagous arthropods (e.g. mosquitoes and ticks) and other blood-sucking parasites (e.g. leeches and worms) could also provide information on vector-borne diseases.
OBJECTIVE: To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid fly. METHODS: Blood meal was recovered from the abdomen of an engorged tabanid fly (Haematopota sp.) which was captured immediately after biting a Sumatran rhino in captivity. The blood was applied on to a Whatman FTA(®) blood card. Subsequent laboratory work was conducted to extract, amplify and sequence the DNA from the sample. Validation was done by sampling the hair follicles and blood samples from the rhinoceros and subjecting it to the same laboratory process. RESULTS: BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from the rhino. CONCLUSIONS: This method could be used in the field application to noninvasively collect genetic samples. Collection of tabanids and other haematophagous arthropods (e.g. mosquitoes and ticks) and other blood-sucking parasites (e.g. leeches and worms) could also provide information on vector-borne diseases.
Entities:
Keywords:
Blood meal; Noninvasive DNA sampling; Sumatran rhino; Tabanid fly
Authors: Ida Bærholm Schnell; Philip Francis Thomsen; Nicholas Wilkinson; Morten Rasmussen; Lars R D Jensen; Eske Willerslev; Mads F Bertelsen; M Thomas P Gilbert Journal: Curr Biol Date: 2012-04-24 Impact factor: 10.834
Authors: Eske Willerslev; M Thomas P Gilbert; Jonas Binladen; Simon Y W Ho; Paula F Campos; Aakrosh Ratan; Lynn P Tomsho; Rute R da Fonseca; Andrei Sher; Tatanya V Kuznetsova; Malgosia Nowak-Kemp; Terri L Roth; Webb Miller; Stephan C Schuster Journal: BMC Evol Biol Date: 2009-05-11 Impact factor: 3.260
Authors: Yu Yang; Lindsey S Garver; Karen M Bingham; Jun Hang; Ryan C Jochim; Silas A Davidson; Jason H Richardson; Richard G Jarman Journal: Am J Trop Med Hyg Date: 2015-09-28 Impact factor: 2.345
Authors: Andrea Egizi; Ellen S Martinsen; Holly Vuong; Kelly I Zimmerman; Ary Faraji; Dina M Fonseca Journal: Ecohealth Date: 2018-09-21 Impact factor: 3.184