Literature DB >> 23592706

Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes.

John P Frampton1, Huilin Shi, Albert Kao, Jack M Parent, Shuichi Takayama.   

Abstract

Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  aqueous two-phase system; cardiomyocyte; dextran; polyethylene glycol; stem cell

Mesh：

Substances：

Year:  2013        PMID： 23592706      PMCID： PMC4103153          DOI： 10.1002/adhm.201300049

Source DB:  PubMed          Journal:  Adv Healthc Mater        ISSN： 2192-2640            Impact factor:   9.933


  22 in total

1.  Digital image analysis of haematopoietic clusters.

Authors:  A Benzinou; Y Hojeij; A-C Roudot
Journal:  Comput Methods Programs Biomed       Date:  2005-02       Impact factor: 5.428

2.  Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation.

Authors:  Catharina Ellerström; Raimund Strehl; Karin Noaksson; Johan Hyllner; Henrik Semb
Journal:  Stem Cells       Date:  2007-03-22       Impact factor: 6.277

3.  Human embryonic stem cells passaged using enzymatic methods retain a normal karyotype and express CD30.

Authors:  Alison Thomson; Davina Wojtacha; Zoë Hewitt; Helen Priddle; Virginie Sottile; Alex Di Domenico; Judy Fletcher; Martin Waterfall; Néstor López Corrales; Ray Ansell; Jim McWhir
Journal:  Cloning Stem Cells       Date:  2008-03

4.  Single cell assay with an automated capillary microinjection system.

Authors:  R Pepperkok; C Schneider; L Philipson; W Ansorge
Journal:  Exp Cell Res       Date:  1988-10       Impact factor: 3.905

5.  Derivation of mesenchymal stem cells from human induced pluripotent stem cells cultured on synthetic substrates.

Authors:  L G Villa-Diaz; S E Brown; Y Liu; A M Ross; J Lahann; J M Parent; P H Krebsbach
Journal:  Stem Cells       Date:  2012-06       Impact factor: 6.277

6.  Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium.

Authors:  Miho Kusuda Furue; Daiki Tateyama; Masaki Kinehara; Jie Na; Tetsuji Okamoto; J Denry Sato
Journal:  In Vitro Cell Dev Biol Anim       Date:  2010-04-29       Impact factor: 2.416

7.  Embryonic stem cell lines derived from human blastocysts.

Authors:  J A Thomson; J Itskovitz-Eldor; S S Shapiro; M A Waknitz; J J Swiergiel; V S Marshall; J M Jones
Journal:  Science       Date:  1998-11-06       Impact factor: 47.728

8.  Automated mechanical passaging: a novel and efficient method for human embryonic stem cell expansion.

Authors:  Alexis Joannides; Christelle Fiore-Hériché; Kristine Westmore; Maeve Caldwell; Alastair Compston; Nicholas Allen; Siddharthan Chandran
Journal:  Stem Cells       Date:  2006-02       Impact factor: 6.277

9.  Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential.

Authors:  Kristi A Hohenstein Elliott; Cory Peterson; Anuradha Soundararajan; Natalia Kan; Brandon Nelson; Sean Spiering; Mark Mercola; Gary R Bright
Journal:  Stem Cells Int       Date:  2012-05-30       Impact factor: 5.443

10.  Culture and maintenance of human embryonic stem cells.

Authors:  Lia Kent
Journal:  J Vis Exp       Date:  2009-12-22       Impact factor: 1.355
View more
  5 in total

1.  Aqueous two-phase system-mediated antibody micropatterning enables multiplexed immunostaining of cell monolayers and tissues.

Authors:  John P Frampton; Michael Tsuei; Joshua B White; Abin T Abraham; Shuichi Takayama
Journal:  Biotechnol J       Date:  2014-08-29       Impact factor: 4.677

Review 2.  Recent developments in multiplexing techniques for immunohistochemistry.

Authors:  Angela R Dixon; Cédric Bathany; Michael Tsuei; Joshua White; Kate F Barald; Shuichi Takayama
Journal:  Expert Rev Mol Diagn       Date:  2015-08-06       Impact factor: 5.225

3.  Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.

Authors:  John P Frampton; Joshua B White; Arlyne B Simon; Michael Tsuei; Sophie Paczesny; Shuichi Takayama
Journal:  Sci Rep       Date:  2014-05-02       Impact factor: 4.379

4.  Patterning bacterial communities on epithelial cells.

Authors:  Mohammed Dwidar; Brendan M Leung; Toshiyuki Yaguchi; Shuichi Takayama; Robert J Mitchell
Journal:  PLoS One       Date:  2013-06-13       Impact factor: 3.240

5.  Bioinspired 3D Culture in Nanoliter Hyaluronic Acid-Rich Core-Shell Hydrogel Microcapsules Isolates Highly Pluripotent Human iPSCs.

Authors:  Jiangsheng Xu; James G Shamul; Nicholas A Staten; Alisa M White; Bin Jiang; Xiaoming He
Journal:  Small       Date:  2021-07-14       Impact factor: 15.153
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.