BACKGROUND: Epithelial permeability is highly dependent upon the integrity of tight junctions, which are cell-cell adhesion complexes located at the apical aspect of the lateral membrane of polarized epithelial cells. We hypothesize that sinonasal epithelial exposure to Der p 1 house dust mite antigen decreases expression of tight junction proteins (TJPs), representing a potential mechanism for increased permeability and presentation of antigens across the sinonasal epithelial layer. METHODS: Confluent cultured primary human sinonasal epithelial cells were exposed to recombinant Der p 1 antigen vs control, and transepithelial resistance measurements were performed over 24 hours. Antibody staining for a panel of TJPs was examined with immunofluorescence/confocal microscopy and Western blotting. Tissue for these experiments was obtained from 4 patients total. RESULTS: Der p 1 exposed sinonasal cells showed a marked decrease in transepithelial resistance when compared to control cells. In addition, results of Western immunoblot and immunofluorescent labeling demonstrated decreased expression of TJPs claudin-1 and junction adhesion molecule-A (JAM-A) in Der p 1-exposed cultured sinonasal cells vs controls. CONCLUSION: Der p 1 antigen exposure decreases sinonasal epithelium TJP expression, most notably seen in JAM-A and claudin-1 in these preliminary experiments. This decreased TJP expression likely contributes to increased epithelial permeability and represents a potential mechanism for transepithelial antigen exposure in allergic rhinitis.
BACKGROUND: Epithelial permeability is highly dependent upon the integrity of tight junctions, which are cell-cell adhesion complexes located at the apical aspect of the lateral membrane of polarized epithelial cells. We hypothesize that sinonasal epithelial exposure to Der p 1 house dust mite antigen decreases expression of tight junction proteins (TJPs), representing a potential mechanism for increased permeability and presentation of antigens across the sinonasal epithelial layer. METHODS: Confluent cultured primary human sinonasal epithelial cells were exposed to recombinant Der p 1 antigen vs control, and transepithelial resistance measurements were performed over 24 hours. Antibody staining for a panel of TJPs was examined with immunofluorescence/confocal microscopy and Western blotting. Tissue for these experiments was obtained from 4 patients total. RESULTS: Der p 1 exposed sinonasal cells showed a marked decrease in transepithelial resistance when compared to control cells. In addition, results of Western immunoblot and immunofluorescent labeling demonstrated decreased expression of TJPs claudin-1 and junction adhesion molecule-A (JAM-A) in Der p 1-exposed cultured sinonasal cells vs controls. CONCLUSION: Der p 1 antigen exposure decreases sinonasal epithelium TJP expression, most notably seen in JAM-A and claudin-1 in these preliminary experiments. This decreased TJP expression likely contributes to increased epithelial permeability and represents a potential mechanism for transepithelial antigen exposure in allergic rhinitis.
Authors: H Wan; H L Winton; C Soeller; E R Tovey; D C Gruenert; P J Thompson; G A Stewart; G W Taylor; D R Garrod; M B Cannell; C Robinson Journal: J Clin Invest Date: 1999-07 Impact factor: 14.808
Authors: H Wan; H L Winton; C Soeller; D C Gruenert; P J Thompson; M B Cannell; G A Stewart; D R Garrod; C Robinson Journal: Clin Exp Allergy Date: 2000-05 Impact factor: 5.018
Authors: C A Herbert; C M King; P C Ring; S T Holgate; G A Stewart; P J Thompson; C Robinson Journal: Am J Respir Cell Mol Biol Date: 1995-04 Impact factor: 6.914
Authors: C Robinson; N A Kalsheker; N Srinivasan; C M King; D R Garrod; P J Thompson; G A Stewart Journal: Clin Exp Allergy Date: 1997-01 Impact factor: 5.018
Authors: Kåre Meno; Peter B Thorsted; Henrik Ipsen; Ole Kristensen; Jørgen N Larsen; Michael D Spangfort; Michael Gajhede; Kaare Lund Journal: J Immunol Date: 2005-09-15 Impact factor: 5.422
Authors: Sarah K Wise; Adrienne M Laury; Elizabeth H Katz; Kyle A Den Beste; Charles A Parkos; Asma Nusrat Journal: Int Forum Allergy Rhinol Date: 2014-02-07 Impact factor: 3.858
Authors: Delicia Shu-Qin Ooi; Cheryl Pei-Ting Tan; Michelle Jia-Yu Tay; Siong Gim Ong; Elizabeth Huiwen Tham; Kewin Tien Ho Siah; Johan Gunnar Eriksson; Keith M Godfrey; Lynette Pei-Chi Shek; Evelyn Xiu-Ling Loo Journal: J Dev Orig Health Dis Date: 2020-06-15 Impact factor: 2.401
Authors: Cristina Gomez-Casado; Javier Sanchez-Solares; Elena Izquierdo; Araceli Díaz-Perales; Domingo Barber; María M Escribese Journal: Foods Date: 2021-04-28
Authors: Siti Muhamad Nur Husna; Che Othman Siti Sarah; Hern-Tze Tina Tan; Norasnieda Md Shukri; Noor Suryani Mohd Ashari; Kah Keng Wong Journal: Sci Rep Date: 2021-01-13 Impact factor: 4.379