BACKGROUND: Red light is part of the visible light spectrum. The effects of light-emitting diode (LED)-generated red light on human skin are not well-characterized. OBJECTIVE: To study the effect of red LED-generated low-level light therapy (LLLT) on fibroblast proliferation and viability in vitro. METHODS AND MATERIALS: Irradiation of normal human skin fibroblasts using red LED panels was performed in vitro, and modulation of proliferation and viability was quantified using trypan blue dye exclusion assay. RESULTS: Statistically significant decreases in cell proliferation were noted at the following fluences (time): 160 J/cm2 (30 minutes, 34 seconds), 320 J/cm2 (61 minutes, 07 seconds) and 640 J/cm2 (122 minutes, 14 seconds) (Figure 1). Irradiation at the 160- (98.5 ± 1.2%) and 320-J/cm2 (98.0 ± 3.1%) doses did not significantly alter viability. CONCLUSION: At certain fluences, red LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars and other proliferative skin diseases.
RCT Entities:
BACKGROUND: Red light is part of the visible light spectrum. The effects of light-emitting diode (LED)-generated red light on human skin are not well-characterized. OBJECTIVE: To study the effect of red LED-generated low-level light therapy (LLLT) on fibroblast proliferation and viability in vitro. METHODS AND MATERIALS: Irradiation of normal human skin fibroblasts using red LED panels was performed in vitro, and modulation of proliferation and viability was quantified using trypan blue dye exclusion assay. RESULTS: Statistically significant decreases in cell proliferation were noted at the following fluences (time): 160 J/cm2 (30 minutes, 34 seconds), 320 J/cm2 (61 minutes, 07 seconds) and 640 J/cm2 (122 minutes, 14 seconds) (Figure 1). Irradiation at the 160- (98.5 ± 1.2%) and 320-J/cm2 (98.0 ± 3.1%) doses did not significantly alter viability. CONCLUSION: At certain fluences, red LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars and other proliferative skin diseases.
Authors: Robson Alexandre Brochetti; Mayara Peres Leal; Raíssa Rodrigues; Renata Kelly da Palma; Luis Vicente Franco de Oliveira; Anna Carolina Ratto Tempestini Horliana; Amílcar Sabino Damazo; Ana Paula Ligeiro de Oliveira; Rodolfo Paula Vieira; Adriana Lino-Dos-Santos-Franco Journal: Lasers Med Sci Date: 2017-07-16 Impact factor: 3.161
Authors: Anna Ossmann; Stefan Kranz; Guellmar Andre; Andrea Völpel; Volker Albrecht; Alfred Fahr; Bernd W Sigusch Journal: Clin Oral Investig Date: 2014-06-21 Impact factor: 3.573
Authors: Alana Kurtti; Julie K Nguyen; Jeremy Weedon; Andrew Mamalis; Yi Lai; Natasha Masub; Amaris Geisler; Daniel M Siegel; Jared R Jagdeo Journal: J Biophotonics Date: 2021-05-04 Impact factor: 3.207