| Literature DB >> 2358423 |
M Abe1, K Takahashi, K Hiwada.
Abstract
A method for the co-purification of calponin and caldesmon from bovine aorta smooth muscle was established involving heat treatment, ammonium sulfate fractionation (0-30 and 30-50%), CM52 column chromatography, and Ultrogel AcA44 gel filtration. The yields of calponin and caldesmon from 200 g of smooth muscle were 17 and 32 mg, respectively. This simplified, fast, and efficient method for the purification of calponin and caldesmon constitutes a useful tool for studying thin filament-linked regulation of smooth muscle contraction.Entities:
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Year: 1990 PMID： 2358423 DOI： 10.1093/oxfordjournals.jbchem.a123075
Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387