| Literature DB >> 23583822 |
Richard B M Schasfoort1, Arthur E H Bentlage, Ivan Stojanovic, Alex van der Kooi, Ellen van der Schoot, Leon W M M Terstappen, Gestur Vidarsson.
Abstract
A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.Mesh：
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Year: 2013 PMID： 23583822 DOI： 10.1016/j.ab.2013.04.001
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365