BACKGROUND: In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated. RESULTS: Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axoneme and constitutes part of the outer-inner dynein (OID) linker. Furthermore, we identified IC2 as a functional hub between ODAs and IDAs based on the phenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella of the IC2 mutant showed activated microtubule sliding and enhanced ATPase activities of ODAs, as well as an altered waveform, indicating attenuated IDA activity. CONCLUSIONS: We concluded that the OID linker controls both ODAs and IDAs and regulates flagellar beating.
BACKGROUND: In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated. RESULTS: Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axoneme and constitutes part of the outer-inner dynein (OID) linker. Furthermore, we identified IC2 as a functional hub between ODAs and IDAs based on the phenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella of the IC2 mutant showed activated microtubule sliding and enhanced ATPase activities of ODAs, as well as an altered waveform, indicating attenuated IDA activity. CONCLUSIONS: We concluded that the OID linker controls both ODAs and IDAs and regulates flagellar beating.
Authors: Lea M Alford; Daniel Stoddard; Jennifer H Li; Emily L Hunter; Douglas Tritschler; Raqual Bower; Daniela Nicastro; Mary E Porter; Winfield S Sale Journal: Cytoskeleton (Hoboken) Date: 2016-06-13
Authors: Mikito Owa; Akane Furuta; Jiro Usukura; Fumio Arisaka; Stephen M King; George B Witman; Ritsu Kamiya; Ken-ichi Wakabayashi Journal: Proc Natl Acad Sci U S A Date: 2014-06-16 Impact factor: 11.205
Authors: Long Gui; Kangkang Song; Douglas Tritschler; Raqual Bower; Si Yan; Aguang Dai; Katherine Augspurger; Jason Sakizadeh; Magdalena Grzemska; Thomas Ni; Mary E Porter; Daniela Nicastro Journal: Proc Natl Acad Sci U S A Date: 2019-10-28 Impact factor: 11.205
Authors: Petra Zur Lage; Zhiyan Xi; Jennifer Lennon; Iain Hunter; Wai Kit Chan; Alfonso Bolado Carrancio; Alex von Kriegsheim; Andrew P Jarman Journal: Biol Open Date: 2021-10-28 Impact factor: 2.422