Ke Chen1, Huacui Xiong, Yibin Huang, Caiqi Liu. 1. Department of Stomatology, Guangzhou Women and Children's Medical Center, 9 Jinsui Road, Guangzhou 510623, PR China. dentchenke@sohu.com
Abstract
OBJECTIVE: The aim of this study was to compare the in vitro periodontal properties of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs). DESIGN: SCAP and PDLSCs cultures were established from normal human impacted third molars with immature roots. The cells were cultured in differentiation medium containing dexamethasone, ß-glycerophosphate and ascorbate phosphate for 3 weeks and in normal medium for as long as 60 days, and then were analysed for mineralisation potential. Cell proliferation, colony-forming capacity and periodontal ligament (PDL)-specific markers were also measured. The mineralisation markers, including alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC), were investigated by immunofluorescence staining and real-time polymerase chain reaction. The expression of PDL markers, including periostin and S100A4, was confirmed by reverse transcription polymerase chain reaction. RESULTS: SCAP showed a significantly higher proliferation rate and colony-forming capacity than PDLSCs. Both types of cells displayed mineralisation potential after induction and long-term culture. The SCAP, however, exhibited higher levels of ALP, BSP and OC expression than the PDLSCs. Like the PDLSCs, the SCAP exhibited periostin and S100A4 expression. CONCLUSIONS: Our study provides the first evidence showing that SCAP express periodontal properties in vitro. SCAP not only showed PDL-related markers, but also displayed a higher proliferation rate and a greater mineralisation capacity than those of PDLSCs. It might help understand the development of tooth root and periodontium. Furthermore, SCAP could be a promising candidate for periodontal tissue engineering.
OBJECTIVE: The aim of this study was to compare the in vitro periodontal properties of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs). DESIGN:SCAP and PDLSCs cultures were established from normal human impacted third molars with immature roots. The cells were cultured in differentiation medium containing dexamethasone, ß-glycerophosphate and ascorbate phosphate for 3 weeks and in normal medium for as long as 60 days, and then were analysed for mineralisation potential. Cell proliferation, colony-forming capacity and periodontal ligament (PDL)-specific markers were also measured. The mineralisation markers, including alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC), were investigated by immunofluorescence staining and real-time polymerase chain reaction. The expression of PDL markers, including periostin and S100A4, was confirmed by reverse transcription polymerase chain reaction. RESULTS:SCAP showed a significantly higher proliferation rate and colony-forming capacity than PDLSCs. Both types of cells displayed mineralisation potential after induction and long-term culture. The SCAP, however, exhibited higher levels of ALP, BSP and OC expression than the PDLSCs. Like the PDLSCs, the SCAP exhibited periostin and S100A4 expression. CONCLUSIONS: Our study provides the first evidence showing that SCAP express periodontal properties in vitro. SCAP not only showed PDL-related markers, but also displayed a higher proliferation rate and a greater mineralisation capacity than those of PDLSCs. It might help understand the development of tooth root and periodontium. Furthermore, SCAP could be a promising candidate for periodontal tissue engineering.