| Literature DB >> 23581642 |
Toru Komatsu1, Kenjiro Hanaoka, Alexander Adibekian, Kentaro Yoshioka, Takuya Terai, Tasuku Ueno, Mitsuyasu Kawaguchi, Benjamin F Cravatt, Tetsuo Nagano.
Abstract
We have established the diced electrophoresis gel (DEG) assay as a proteome-wide screening tool to identify enzymes with activities of interest using turnover-based fluorescent substrates. The method utilizes the combination of native polyacrylamide gel electrophoresis (PAGE) with a multiwell-plate-based fluorometric assay to find protein spots with the specified activity. By developing fluorescent substrates that mimic the structure of neutrophil chemoattractants, we could identify enzymes involved in metabolic inactivation of the chemoattractants.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23581642 DOI： 10.1021/ja401792d
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419