CONTEXT: Chronic liver disease has become a global health problem. The research for prominent herbal agents for the management of liver diseases is widely increased. OBJECTIVE: The root of Abelmoschus esculentus (Linn.) Moench., (Malvaceae) has been used as a remedy for liver disorders. The aim of the present study was to evaluate the antioxidant and hepatoprotective effects of the ethanol extract of A. esculentus root. MATERIALS AND METHOD: The antioxidant effect was assessed using DPPH and hydroxy radical scavenging assays. The hepatoprotective effect of the extract was evaluated using CCl₄ intoxicated HepG₂ cell line and Wistar rats by estimating the levels of hepatic and antioxidant markers. RESULTS: The extract of A. esculentus showed IC₅₀ values of 270.99 and 532.86 µg/mL for DPPH and hydroxy radical scavenging assays, respectively. The incubation of HepG2 cells with CCl₄ drastically decreased the cell viability and increased the leakage of transaminases. Pre-treatment with the extract significantly restored the cell death by 31.25 and 39.04% at 200 and 400 µg/mL concentrations, respectively. The reduction of ALT leakage by the treatment was 18.62, 38.59 and 52.15% compared to the CCl₄ treated cells at 100, 200 and 400 µg/mL, respectively. In in-vivo experiments also the treatment reduced the levels of transaminases, ALP, MDA, total bilirubin and hepatic TNFα levels as well as increased the antioxidant levels in a dose dependent manner. Histological observations of liver sections showed reduction in steatosis, necrosis and inflammation. CONCLUSION: The results substantiated the hepatoprotective activity of A. esculentus through its antioxidant capacity.
CONTEXT: Chronic liver disease has become a global health problem. The research for prominent herbal agents for the management of liver diseases is widely increased. OBJECTIVE: The root of Abelmoschus esculentus (Linn.) Moench., (Malvaceae) has been used as a remedy for liver disorders. The aim of the present study was to evaluate the antioxidant and hepatoprotective effects of the ethanol extract of A. esculentus root. MATERIALS AND METHOD: The antioxidant effect was assessed using DPPH and hydroxy radical scavenging assays. The hepatoprotective effect of the extract was evaluated using CCl₄ intoxicated HepG₂ cell line and Wistar rats by estimating the levels of hepatic and antioxidant markers. RESULTS: The extract of A. esculentus showed IC₅₀ values of 270.99 and 532.86 µg/mL for DPPH and hydroxy radical scavenging assays, respectively. The incubation of HepG2 cells with CCl₄ drastically decreased the cell viability and increased the leakage of transaminases. Pre-treatment with the extract significantly restored the cell death by 31.25 and 39.04% at 200 and 400 µg/mL concentrations, respectively. The reduction of ALT leakage by the treatment was 18.62, 38.59 and 52.15% compared to the CCl₄ treated cells at 100, 200 and 400 µg/mL, respectively. In in-vivo experiments also the treatment reduced the levels of transaminases, ALP, MDA, total bilirubin and hepatic TNFα levels as well as increased the antioxidant levels in a dose dependent manner. Histological observations of liver sections showed reduction in steatosis, necrosis and inflammation. CONCLUSION: The results substantiated the hepatoprotective activity of A. esculentus through its antioxidant capacity.
Authors: S S Hasson; A S H Al-Shubi; J Z Al-Busaidi; M S Al-Balushi; F L Hakkim; L Rashan; G M Aleemallah; A A Al-Jabri Journal: Asian Pac J Cancer Prev Date: 2018-07-27