BACKGROUND: Dengue virus ( DENV) nonstructural protein 1 ( NS1) has been used as a novel diagnostic marker during the early phase of DENV infection. METHODS: Presence of NS1 antigen was examined using 336 serum samples obtained from 276 travelers returning to Japan from Asia, Central and South America, Pacific Islands, and Africa with dengue. Assay specificity was evaluated using 148 non-dengue samples. RESULTS: Positive rates among four DENV serotypes were 68%-89%. NS1 antigen positive rates were at similar levels between primary infection and secondary infection. NS1 antigen positive rates were 88%-96% on days 1-5, 75%-100% on days 6-10, and 36-60% on ≥ day 11. Positive rates using real-time polymerase chain reaction (RT-PCR) were over 70% on days 1-5, but decreased thereafter. CONCLUSIONS: The results indicate that NS1 antigen positive rates were higher than those of RT-PCR during longer period of early phase in DENV infection. Thus, NS1 antigen ELISA is a useful tool for confirming DENV infection in international travelers, when it is used in combination with anti-DENV IgM ELISA.
BACKGROUND:Dengue virus ( DENV) nonstructural protein 1 ( NS1) has been used as a novel diagnostic marker during the early phase of DENVinfection. METHODS: Presence of NS1 antigen was examined using 336 serum samples obtained from 276 travelers returning to Japan from Asia, Central and South America, Pacific Islands, and Africa with dengue. Assay specificity was evaluated using 148 non-dengue samples. RESULTS: Positive rates among four DENV serotypes were 68%-89%. NS1 antigen positive rates were at similar levels between primary infection and secondary infection. NS1 antigen positive rates were 88%-96% on days 1-5, 75%-100% on days 6-10, and 36-60% on ≥ day 11. Positive rates using real-time polymerase chain reaction (RT-PCR) were over 70% on days 1-5, but decreased thereafter. CONCLUSIONS: The results indicate that NS1 antigen positive rates were higher than those of RT-PCR during longer period of early phase in DENVinfection. Thus, NS1 antigen ELISA is a useful tool for confirming DENVinfection in international travelers, when it is used in combination with anti-DENV IgM ELISA.
Authors: Ralph Huits; Patrick Soentjens; Ula Maniewski-Kelner; Caroline Theunissen; Steven Van Den Broucke; Eric Florence; Jan Clerinx; Erika Vlieghe; Jan Jacobs; Lieselotte Cnops; Dorien Van Den Bossche; Marjan Van Esbroeck; Emmanuel Bottieau Journal: Open Forum Infect Dis Date: 2017-01-09 Impact factor: 3.835
Authors: Priscila Conrado Guerra Nunes; Rita Maria Ribeiro Nogueira; Manoela Heringer; Thaís Chouin-Carneiro; Cintia Damasceno Dos Santos Rodrigues; Ana Maria Bispo de Filippis; Monique da Rocha Queiroz Lima; Flávia Barreto Dos Santos Journal: Viruses Date: 2018-06-14 Impact factor: 5.048