Literature DB >> 23573044

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India.

Shivani Bisht1, N S Bisht, Snehlata Bhandari.   

Abstract

An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl(-1)) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5-1.0 mgl(-1)) auxins (NAA/IBA/IAA). 1.0 mgl(-1) BAP with 0.5 mgl(-1) NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl(-1) NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.

Entities:  

Keywords:  Liliaceae; Meda/Mahameda; Micropropagation

Year:  2011        PMID： 23573044      PMCID： PMC3550528          DOI： 10.1007/s12298-011-0091-5

Source DB:  PubMed          Journal:  Physiol Mol Biol Plants        ISSN： 0974-0430


  2 in total

1.  In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil).

Authors:  S Pattnaik; P K Chand
Journal:  Plant Cell Rep       Date:  1996-08       Impact factor: 4.570

2.  In vitro multiplication and field establishment of Adhatoda beddomei C. B. Clarke, a rare medicinal plant.

Authors:  C G Sudha; S Seeni
Journal:  Plant Cell Rep       Date:  1994-01       Impact factor: 4.570
  2 in total
  3 in total

1.  An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats.

Authors:  J J Chavan; A S Nalawade; N B Gaikwad; R V Gurav; G B Dixit; S R Yadav
Journal:  Physiol Mol Biol Plants       Date:  2014-06-07

2.  In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant.

Authors:  Sompop Prathanturarug; Rungthipwan Pheakkoet; Thaya Jenjittikul; Wongsatit Chuakul; Promchit Saralamp
Journal:  Physiol Mol Biol Plants       Date:  2012-07

3.  Isolation and characterization of quinine from Polygonatum verticillatum: A new marker approach to identify substitution and adulteration.

Authors:  Jaswinder Kaur Virk; Sanjiv Kumar; Ranjit Singh; Avinash C Tripathi; Shailendra K Saraf; Vikas Gupta; Parveen Bansal
Journal:  J Adv Pharm Technol Res       Date:  2016 Oct-Dec
  3 in total

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