Literature DB >> 2357226

A model of nitrogen flow by malonamate in Rhizobium japonicum-soybean symbiosis.

Y S Kim1, H Z Chae.   

Abstract

Two types of novel malonamidases were found in soybean nodules. One (E1) catalyzes the formation of malonamate from malonate and its hydrolysis to ammonia, whereas the other (E2) acts mainly on the hydrolysis of malonamate. E1 and E2 were found in bacteroids, but only E2 was found in the plant cytosol of the nodule. The substrate requirements of E1 and E2 were highly specific for malonate and malonamate, respectively. From these and other results reported previously, we propose that malonamate plays an important role as a nitrogen carrier in the Rhizobium legume symbiosis.

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Year:  1990        PMID: 2357226     DOI: 10.1016/0006-291x(90)90386-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  5 in total

1.  Structure of malonamidase E2 reveals a novel Ser-cisSer-Lys catalytic triad in a new serine hydrolase fold that is prevalent in nature.

Authors:  Sejeong Shin; Tae-Hee Lee; Nam-Chul Ha; Hyun Min Koo; So-Yeon Kim; Heung-Soo Lee; Yu Sam Kim; Byung-Ha Oh
Journal:  EMBO J       Date:  2002-06-03       Impact factor: 11.598

2.  The active site and substrates binding mode of malonyl-CoA synthetase determined by transferred nuclear Overhauser effect spectroscopy, site-directed mutagenesis, and comparative modeling studies.

Authors:  J W Jung; J H An; K B Na; Y S Kim; W Lee
Journal:  Protein Sci       Date:  2000-07       Impact factor: 6.725

3.  New substrates for the dicarboxylate transport system of Sinorhizobium meliloti.

Authors:  S Yurgel; M W Mortimer; K N Rogers; M L Kahn
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

4.  Identification of active-site residues in Bradyrhizobium japonicum malonamidase E2.

Authors:  H M Koo; S O Choi; H M Kim; Y S Kim
Journal:  Biochem J       Date:  2000-07-15       Impact factor: 3.857

5.  Steady-state kinetics of malonyl-CoA synthetase from Bradyrhizobium japonicum and evidence for malonyl-AMP formation in the reaction.

Authors:  Y S Kim; S W Kang
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

  5 in total

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