Literature DB >> 2357221

Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.

J Glauder1, H Ragg, J Rauch, J W Engels.   

Abstract

We have identified a cDNA encoding human peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) with a total length of 3748 bp by screening of a human thyroid carcinoma lambda gt11 library using two heterologous oligonucleotides to conserved regions which derived from frog skin and bovine pituitary PAM sequences. Furthermore we have identified a sequence which differs in a 321 bp deletion. COS cells transfected with a truncated form of this cDNA (lacking the putative carboxyl-terminal transmembrane domain) generated a functional PAM that showed a 20-fold increase of the activity compared to the control and was visualized by immunoblotting.

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Year:  1990        PMID： 2357221     DOI： 10.1016/0006-291x(90)90366-u

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

1.  New nucleotide sequence data on the EMBL file server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

Review 2.  Peptidylgycine α-amidating monooxygenase and copper: a gene-nutrient interaction critical to nervous system function.

Authors:  Danielle Bousquet-Moore; Richard E Mains; Betty A Eipper
Journal:  J Neurosci Res       Date:  2010-09       Impact factor: 4.164

Review 3.  POMC: The Physiological Power of Hormone Processing.

Authors:  Erika Harno; Thanuja Gali Ramamoorthy; Anthony P Coll; Anne White
Journal:  Physiol Rev       Date:  2018-10-01       Impact factor: 37.312

4.  Expression of peptidyl-glycine alpha-amidating mono-oxygenase (PAM) enzymes in morphological abnormalities adjacent to pulmonary tumors.

Authors:  A Martínez; A M Treston; L Saldise; L M Montuenga; R I Linnoila
Journal:  Am J Pathol       Date:  1996-08       Impact factor: 4.307

5.  Neutralization of adrenomedullin inhibits the growth of human glioblastoma cell lines in vitro and suppresses tumor xenograft growth in vivo.

Authors:  L'Houcine Ouafik; Samantha Sauze; Françoise Boudouresque; Olivier Chinot; Christine Delfino; Frédéric Fina; Vincent Vuaroqueaux; Christophe Dussert; Jacqueline Palmari; Henri Dufour; François Grisoli; Pierre Casellas; Nils Brünner; Pierre-Marie Martin
Journal:  Am J Pathol       Date:  2002-04       Impact factor: 4.307

6.  Peptidylglycine alpha-amidating monooxygenase (PAM) immunoreactivity and messenger RNA in human pituitary and increased expression in pituitary tumours.

Authors:  J H Steel; A Martínez; D R Springall; A M Treston; F Cuttitta; J M Polak
Journal:  Cell Tissue Res       Date:  1994-04       Impact factor: 5.249

7.  Coordination of peroxide to the Cu(M) center of peptidylglycine α-hydroxylating monooxygenase (PHM): structural and computational study.

Authors:  Katarzyna Rudzka; Diego M Moreno; Betty Eipper; Richard Mains; Dario A Estrin; L Mario Amzel
Journal:  J Biol Inorg Chem       Date:  2012-12-18       Impact factor: 3.358

8.  Determinants in the cytoplasmic domain of P-selectin required for sorting to secretory granules.

Authors:  P W Modderman; E A Beuling; L A Govers; J Calafat; H Janssen; A E Von dem Borne; A Sonnenberg
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857

Review 9.  Peptidylglycine alpha-amidating monooxygenase: a multifunctional protein with catalytic, processing, and routing domains.

Authors:  B A Eipper; S L Milgram; E J Husten; H Y Yun; R E Mains
Journal:  Protein Sci       Date:  1993-04       Impact factor: 6.725

10.  Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture.

Authors:  K Suzuki; H Shimoi; Y Iwasaki; T Kawahara; Y Matsuura; Y Nishikawa
Journal:  EMBO J       Date:  1990-12       Impact factor: 11.598
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