A N Saiful1, M S Lau, S Sulaiman, O Hidayatulfathi. 1. Biomedical Science Programme, Faculty of Health Science, Universiti Kebangsaan Malaysia, Kampus Kuala Lumpur, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Abstract
OBJECTIVE: To evaluate the effectiveness and residual effects of trypsin modulating oostatic factor-Bacillus thuringiensis israeliensis (TMOF-Bti) formulations against Aedes aegypti (Ae. aegypti) (L.) larvae at UKM Campus Kuala Lumpur. METHODS: Twenty first instar Ae. aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried leaf powder as their source of food. Combination of TMOF-Bti in rice husk formulation with the following weights viz 10, 25, 50 and 100 mg, respectively in duplicate was distributed in the buckets; while TMOF-Bti in wettable powder formulation each weighing viz 2, 5, 10 and 20 mg, respectively in duplicate was also placed in the buckets. The control buckets run in duplicate with 4 L of water and 20 first instar Ae. aegypti larvae. All buckets were covered with mosquito netting. Larval mortality was recorded after 24 hours and weekly for five weeks. A new batch of 20 1(st) instar larvae Ae. aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder. The experiment was repeated for four times. RESULTS: The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied. The TMOF (2%) + Bti (2%) had a good residual effect until the end of 3(rd) week, TMOF (4%) + Bti (4%) until 4(th) week, wettable powder TMOF (20%) + Bti (20%) until the third week. CONCLUSIONS: From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.
OBJECTIVE: To evaluate the effectiveness and residual effects of trypsin modulating oostatic factor-Bacillus thuringiensis israeliensis (TMOF-Bti) formulations against Aedes aegypti (Ae. aegypti) (L.) larvae at UKM Campus Kuala Lumpur. METHODS: Twenty first instar Ae. aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried leaf powder as their source of food. Combination of TMOF-Bti in rice husk formulation with the following weights viz 10, 25, 50 and 100 mg, respectively in duplicate was distributed in the buckets; while TMOF-Bti in wettable powder formulation each weighing viz 2, 5, 10 and 20 mg, respectively in duplicate was also placed in the buckets. The control buckets run in duplicate with 4 L of water and 20 first instar Ae. aegypti larvae. All buckets were covered with mosquito netting. Larval mortality was recorded after 24 hours and weekly for five weeks. A new batch of 20 1(st) instar larvae Ae. aegypti was introduced into each bucket weekly without additional TMOF-Btirice husk formulation or wettable powder. The experiment was repeated for four times. RESULTS: The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied. The TMOF (2%) + Bti (2%) had a good residual effect until the end of 3(rd) week, TMOF (4%) + Bti (4%) until 4(th) week, wettable powder TMOF (20%) + Bti (20%) until the third week. CONCLUSIONS: From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.
Authors: M Keller; B Sneh; N Strizhov; E Prudovsky; A Regev; C Koncz; J Schell; A Zilberstein Journal: Insect Biochem Mol Biol Date: 1996-04 Impact factor: 4.714
Authors: H L Lee; C D Chen; S Mohd Masri; Y F Chiang; K H Chooi; S Benjamin Journal: Southeast Asian J Trop Med Public Health Date: 2008-07 Impact factor: 0.267