OBJECTIVE: To assess anti-psoriatic activity of the methanol extract and the isolated flavonoid quercetin from the rhizome of Smilax china (S. china) Linn. METHODS: Mouse tail test was used for the evaluation of anti-psoriatic activity. Methanol extract (100 and 200 mg/kg b.w.) and isolated flavonoid quercetin (25 and 50 mg/kg b.w.) were tested in Swiss albino mice. Parameters studied in the mouse tail test were changes in epidermal thickness and percentage orthokeratotic values. The anti-inflammatory role of the methanol extract and isolated flavonoid quercetin was evaluated using carrageenan-induced pleurisy in rats. In vitro antiproliferant assay on HaCaT cell lines was also carried out. RESULTS: The isolated flavonoid quercetin from the rhizome of S. china produced significant orthokeratosis (P<0.01) in the mouse tail test. In epidermal thickness, a significant reduction with respect to control was observed in groups treated with retinoic acid and isolated flavonoid quercetin. The methanol extract (200 mg/kg) and isolated flavonoid quercetin (50 mg/kg) showed anti-inflammatory effect in terms of significant inhibition (P<0.001) in leukocyte migration. Maximum antiproliferant activity was shown by isolated flavonoid quercetin (IC50, 62.42±10.20 µg/mL). CONCLUSIONS: From the above data, the flavonoid quercetin shows significant orthokeratosis, anti-inflammatory and maximum antiproliferant activities. To our knowledge, this is the first report on the anti-psoriatic effect of the flavonoid quercetin which is promising for further investigations to prove its anti-psoriatic activity.
OBJECTIVE: To assess anti-psoriatic activity of the methanol extract and the isolated flavonoidquercetin from the rhizome of Smilax china (S. china) Linn. METHODS:Mouse tail test was used for the evaluation of anti-psoriatic activity. Methanol extract (100 and 200 mg/kg b.w.) and isolated flavonoidquercetin (25 and 50 mg/kg b.w.) were tested in Swiss albino mice. Parameters studied in the mouse tail test were changes in epidermal thickness and percentage orthokeratotic values. The anti-inflammatory role of the methanol extract and isolated flavonoidquercetin was evaluated using carrageenan-induced pleurisy in rats. In vitro antiproliferant assay on HaCaT cell lines was also carried out. RESULTS: The isolated flavonoidquercetin from the rhizome of S. china produced significant orthokeratosis (P<0.01) in the mouse tail test. In epidermal thickness, a significant reduction with respect to control was observed in groups treated with retinoic acid and isolated flavonoidquercetin. The methanol extract (200 mg/kg) and isolated flavonoidquercetin (50 mg/kg) showed anti-inflammatory effect in terms of significant inhibition (P<0.001) in leukocyte migration. Maximum antiproliferant activity was shown by isolated flavonoidquercetin (IC50, 62.42±10.20 µg/mL). CONCLUSIONS: From the above data, the flavonoidquercetin shows significant orthokeratosis, anti-inflammatory and maximum antiproliferant activities. To our knowledge, this is the first report on the anti-psoriatic effect of the flavonoidquercetin which is promising for further investigations to prove its anti-psoriatic activity.
Authors: H Yoshida; T Ishikawa; H Hosoai; M Suzukawa; M Ayaori; T Hisada; S Sawada; A Yonemura; K Higashi; T Ito; K Nakajima; T Yamashita; K Tomiyasu; M Nishiwaki; F Ohsuzu; H Nakamura Journal: Biochem Pharmacol Date: 1999-12-01 Impact factor: 5.858
Authors: R L Lipnick; J A Cotruvo; R N Hill; R D Bruce; K A Stitzel; A P Walker; I Chu; M Goddard; L Segal; J A Springer Journal: Food Chem Toxicol Date: 1995-03 Impact factor: 6.023
Authors: Maicon Roberto Kviecinski; Isabela Machado Barbosa David; Flávia de Souza Fernandes; Marina Dos Reis Correa; Morgana Miranda Clarinda; Amanda Fernandes Freitas; Jane da Silva; Marta Gava; Simony Davet Müller; Drielly Florentino; Fabrícia Petronilho; Diego Moterle; Luiz Alberto Kanis; Rozangela Curi Pedrosa Journal: Pharm Biol Date: 2017-12 Impact factor: 3.503