OBJECTIVE: To investigate the relationship between polymorphism within the 5'-untranslated region (5'-UTR) of IGF-I gene and its periparturient concentration in Iranian Holstein dairy cows. METHODS: Blood samples (5 mL, n = 37) were collected by caudal venipuncture from each animal into sample tubes containing the EDTA and DNA was extracted from blood. In order to measure IGF-I concentration the collection of blood samples (n = 111) was also done at 14 d before calving (prepartum), 25 and 45 d postpartum. RESULTS: We found evidence for a significant effect of C to T mutation in position 512 of IGF-I gene on its serum concentration in dairy cows in Iran. Cows with CC genotype had significantly higher concentration (Mean±SD) of IGF-I at 14 d prepartum (91.8±18.1) µg/L compared to those with TT genotype (73.3±14.4) µg/L (P=0.04). A significant trend (quadratic) was found for IGF-I concentration, as higher in CC cows compared to ones with TT genotype, during the 14 d before calving to 45 d postpartum (P=0.01). CONCLUSIONS: We concluded that C/T transition in the promoter region of IGF-I gene can influence the serum concentration of IGF-I in periparturient dairy cows.
OBJECTIVE: To investigate the relationship between polymorphism within the 5'-untranslated region (5'-UTR) of IGF-I gene and its periparturient concentration in Iranian Holstein dairy cows. METHODS: Blood samples (5 mL, n = 37) were collected by caudal venipuncture from each animal into sample tubes containing the EDTA and DNA was extracted from blood. In order to measure IGF-I concentration the collection of blood samples (n = 111) was also done at 14 d before calving (prepartum), 25 and 45 d postpartum. RESULTS: We found evidence for a significant effect of C to T mutation in position 512 of IGF-I gene on its serum concentration in dairy cows in Iran. Cows with CC genotype had significantly higher concentration (Mean±SD) of IGF-I at 14 d prepartum (91.8±18.1) µg/L compared to those with TT genotype (73.3±14.4) µg/L (P=0.04). A significant trend (quadratic) was found for IGF-I concentration, as higher in CC cows compared to ones with TT genotype, during the 14 d before calving to 45 d postpartum (P=0.01). CONCLUSIONS: We concluded that C/T transition in the promoter region of IGF-I gene can influence the serum concentration of IGF-I in periparturient dairy cows.
Authors: Jerônimo Rubert Stefanello; Marcos Henrique Barreta; Patricia Marafon Porciuncula; João Nelson Arruda; João Francisco Oliveira; Marcos Antonio Oliveira; Paulo Bayard Gonçalves Journal: Theriogenology Date: 2006-08-04 Impact factor: 2.740
Authors: M A Velazquez; M Newman; M F Christie; P J Cripps; M A Crowe; R F Smith; H Dobson Journal: Theriogenology Date: 2005-06-04 Impact factor: 2.740
Authors: Mohammed A El-Magd; Ayman A Saleh; Abeer A Nafeaa; Shymaa M El-Komy; Mohamed A Afifi Journal: J Zhejiang Univ Sci B Date: 2017 Dec. Impact factor: 3.066