OBJECTIVE: To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi. METHODS: The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) and chloroform soluble fraction (5.0 g). The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method. Commercially available kanamycin (30 µg/disc) was used as standard disc and blank discs impregnated with the respective solvents were used as negative control. RESULTS: At a concentration of 400 µg/disc, all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm. Among the tested samples, the crude ethanol extract showed the highest activity against Vibrio parahemolyticus (V. parahemolyticus). The minimum inhibitory concentration (MIC) of the crude ethanol extract and its fractions were within the value of 128-256 µg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V. parahemolyticus (128 µg/mL). CONCLUSIONS: It can be concluded that, potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z. zerumbet (L).
OBJECTIVE: To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi. METHODS: The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) and chloroform soluble fraction (5.0 g). The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method. Commercially available kanamycin (30 µg/disc) was used as standard disc and blank discs impregnated with the respective solvents were used as negative control. RESULTS: At a concentration of 400 µg/disc, all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm. Among the tested samples, the crude ethanol extract showed the highest activity against Vibrio parahemolyticus (V. parahemolyticus). The minimum inhibitory concentration (MIC) of the crude ethanol extract and its fractions were within the value of 128-256 µg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V. parahemolyticus (128 µg/mL). CONCLUSIONS: It can be concluded that, potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z. zerumbet (L).
Authors: Z A Zakaria; A S Mohamad; M S Ahmad; A F Mokhtar; D A Israf; N H Lajis; M R Sulaiman Journal: Biol Res Nurs Date: 2010-11-26 Impact factor: 2.522
Authors: N J Yob; S Mohd Jofrry; M M R Meor Mohd Affandi; L K Teh; M Z Salleh; Z A Zakaria Journal: Evid Based Complement Alternat Med Date: 2011-03-22 Impact factor: 2.629
Authors: Heshu Sulaiman Rahman; Abdullah Rasedee; Swee Keong Yeap; Hemn Hassan Othman; Max Stanley Chartrand; Farideh Namvar; Ahmad Bustamam Abdul; Chee Wun How Journal: Biomed Res Int Date: 2014-06-16 Impact factor: 3.411
Authors: Nabilah Muhammad Nadzri; Ahmad Bustamam Abdul; Mohd Aspollah Sukari; Siddig Ibrahim Abdelwahab; Eltayeb E M Eid; Syam Mohan; Behnam Kamalidehghan; Theebaa Anasamy; Kuan Beng Ng; Suvitha Syam; Ismail Adam Arbab; Heshu Sulaiman Rahman; Hapipah Mohd Ali Journal: Evid Based Complement Alternat Med Date: 2013-05-08 Impact factor: 2.629
Authors: Ali Ghasemzadeh; Hawa Z E Jaafar; Sadegh Ashkani; Asmah Rahmat; Abdul Shukor Juraimi; Adam Puteh; Mahmud Tengku Muda Mohamed Journal: BMC Complement Altern Med Date: 2016-03-22 Impact factor: 3.659