PURPOSE: Quantitative measure of blood flow provides important information regarding renal function, nephropathies and viability of kidney transplantation. Therefore, a method that would allow quantitative and reliable assessment of the renal microvascular perfusion would be very valuable. Arterial spin labeling Magnetic Resonance Imaging has started to be widely used for human studies. For rodents though, despite the increasing number of transgenic mouse models, renal perfusion Magnetic Resonance Imaging has been only sparsely reported. This study investigated the use of FAIR (flow-sensitive alternating inversion recovery) and pseudo-continuous arterial spin labeling (pCASL) for mouse renal blood flow measurements. METHODS: FAIR and pCASL were compared in terms of sensitivity, absolute quantification, reproducibility and flexibility of implementation. Multislice and coronal imaging were also investigated. Studies were performed at 11.75 T with volumic transmitter/receiver radiofrequency coils and fast imaging. RESULTS: pCASL demonstrated better experimental flexibility and higher sensitivity compared to FAIR (> +20%). Renal blood flow values in the range of 550-750 mL/100 g/min for the cortex and of 140-230 mL/100 g/min for the medulla, consistent with literature data, were measured. CONCLUSION: pCASL was successfully applied at very high field for mouse renal blood flow measurements, demonstrating high sensitivity, flexibility and multislice imaging capability. pCASL may be considered as a method of choice for mouse kidney perfusion studies.
PURPOSE: Quantitative measure of blood flow provides important information regarding renal function, nephropathies and viability of kidney transplantation. Therefore, a method that would allow quantitative and reliable assessment of the renal microvascular perfusion would be very valuable. Arterial spin labeling Magnetic Resonance Imaging has started to be widely used for human studies. For rodents though, despite the increasing number of transgenic mouse models, renal perfusion Magnetic Resonance Imaging has been only sparsely reported. This study investigated the use of FAIR (flow-sensitive alternating inversion recovery) and pseudo-continuous arterial spin labeling (pCASL) for mouse renal blood flow measurements. METHODS: FAIR and pCASL were compared in terms of sensitivity, absolute quantification, reproducibility and flexibility of implementation. Multislice and coronal imaging were also investigated. Studies were performed at 11.75 T with volumic transmitter/receiver radiofrequency coils and fast imaging. RESULTS: pCASL demonstrated better experimental flexibility and higher sensitivity compared to FAIR (> +20%). Renal blood flow values in the range of 550-750 mL/100 g/min for the cortex and of 140-230 mL/100 g/min for the medulla, consistent with literature data, were measured. CONCLUSION: pCASL was successfully applied at very high field for mouse renal blood flow measurements, demonstrating high sensitivity, flexibility and multislice imaging capability. pCASL may be considered as a method of choice for mouse kidney perfusion studies.
Authors: Celine Baligand; Hecong Qin; Aisha True-Yasaki; Jeremy W Gordon; Cornelius von Morze; Justin Delos Santos; David M Wilson; Robert Raffai; Patrick M Cowley; Anthony J Baker; John Kurhanewicz; David H Lovett; Zhen Jane Wang Journal: NMR Biomed Date: 2017-07-14 Impact factor: 4.044
Authors: Cesar A Romero; Glauber Cabral; Robert A Knight; Guangliang Ding; Edward L Peterson; Oscar A Carretero Journal: Am J Physiol Renal Physiol Date: 2017-10-04
Authors: Ying Gao; Candida L Goodnough; Bernadette O Erokwu; George W Farr; Rebecca Darrah; Lan Lu; Katherine M Dell; Xin Yu; Chris A Flask Journal: NMR Biomed Date: 2014-06-03 Impact factor: 4.044
Authors: R Ramasawmy; A E Campbell-Washburn; J A Wells; S P Johnson; R B Pedley; S Walker-Samuel; M F Lythgoe Journal: NMR Biomed Date: 2014-12-17 Impact factor: 4.044