BACKGROUND: The health risks of crack cocaine smoking on the oral mucosa has not been widely researched and documented. OBJECTIVE: The purpose of this study was to analyze the proliferative activity of oral epithelial cells exposed to crack cocaine smoke using silver nucleolar organizer region (AgNOR) staining. METHODS: Oral smears were collected from clinically normal-appearing buccal mucosa by liquid-based exfoliative cytology of 60 individuals (30 crack cocaine users and 30 healthy controls matched for age and gender) and analyzed for cytomorphologic and cytomorphometric techniques. RESULTS: Crack cocaine users consumed about 13.3 heat-stable rocks per day and the time consumption of the drug was of 5.2 (± 3.3) years. Mean values of AgNOR counting for case and control groups were 5.18 ± 1.83 and 3.38 ± 1.02 (P<0.05), respectively. AgNOR area and percentage of AgNOR-occupied nuclear area were increased in comparison with the control (P<0.05). There was no statistically significant difference in the mean values of the nuclear area between the groups (P>0.05). CONCLUSION: This study revealed that crack cocaine smoke increases the rate of cellular proliferation in cells of normal buccal mucosa.
BACKGROUND: The health risks of crack cocaine smoking on the oral mucosa has not been widely researched and documented. OBJECTIVE: The purpose of this study was to analyze the proliferative activity of oral epithelial cells exposed to crack cocaine smoke using silver nucleolar organizer region (AgNOR) staining. METHODS: Oral smears were collected from clinically normal-appearing buccal mucosa by liquid-based exfoliative cytology of 60 individuals (30 crack cocaine users and 30 healthy controls matched for age and gender) and analyzed for cytomorphologic and cytomorphometric techniques. RESULTS:Crack cocaine users consumed about 13.3 heat-stable rocks per day and the time consumption of the drug was of 5.2 (± 3.3) years. Mean values of AgNOR counting for case and control groups were 5.18 ± 1.83 and 3.38 ± 1.02 (P<0.05), respectively. AgNOR area and percentage of AgNOR-occupied nuclear area were increased in comparison with the control (P<0.05). There was no statistically significant difference in the mean values of the nuclear area between the groups (P>0.05). CONCLUSION: This study revealed that crack cocaine smoke increases the rate of cellular proliferation in cells of normal buccal mucosa.
Authors: João Adolfo Costa Hanemann; Marta Miyazawa; Mireile São Geraldo dos Santos Souza Journal: J Appl Oral Sci Date: 2011 May-Jun Impact factor: 2.698
Authors: Maria das Graças Alonso de Oliveira; Jean Nunes Dos Santos; Patrícia Ramos Cury; Victor Hugo Pereira da Silva; Nara Rejane Cruz Oliveira; Ricardo da Costa Padovani; Adriana Marcassa Tucci; Daniel Araki Ribeiro Journal: Environ Sci Pollut Res Int Date: 2014-01-16 Impact factor: 4.223
Authors: Patricia R Cury; Maria G A Oliveira; Katia Montanha de Andrade; Maíra D S de Freitas; Jean N Dos Santos Journal: Environ Sci Pollut Res Int Date: 2017-01-24 Impact factor: 4.223