Clare L Hawkins1, Michael J Davies. 1. The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia; Faculty of Medicine, Sydney Medical School, Sydney, NSW 2006, Australia.
Abstract
BACKGROUND: Electron paramagnetic resonance (EPR) spectroscopy (also known as electron spin resonance, ESR, spectroscopy) is widely considered to be the "gold standard" for the detection and characterisation of radicals in biological systems. SCOPE OF REVIEW: The article reviews the major positive and negative aspects of EPR spectroscopy and discusses how this technique and associated methodologies can be used to maximise useful information, and minimise artefacts, when used in biological studies. Consideration is given to the direct detection of radicals (at both ambient and low temperature), the use of spin trapping and spin scavenging (e.g. reaction with hydroxylamines), the detection of nitric oxide and the detection and quantification of some transition metal ions (particularly iron and copper) and their environment. MAJOR CONCLUSIONS: When used with care this technique can provide a wealth of valuable information on the presence of radicals and some transition metal ions in biological systems. It can provide definitive information on the identity of the species present and also information on their concentration, structure, mobility and interactions. It is however a technique that has major limitations and the user needs to understand the various pitfalls and shortcoming of the method to avoid making errors. GENERAL SIGNIFICANCE: EPR remains the most definitive method of identifying radicals in complex systems and is also a valuable method of examining radical kinetics, concentrations and structure. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
BACKGROUND: Electron paramagnetic resonance (EPR) spectroscopy (also known as electron spin resonance, ESR, spectroscopy) is widely considered to be the "gold standard" for the detection and characterisation of radicals in biological systems. SCOPE OF REVIEW: The article reviews the major positive and negative aspects of EPR spectroscopy and discusses how this technique and associated methodologies can be used to maximise useful information, and minimise artefacts, when used in biological studies. Consideration is given to the direct detection of radicals (at both ambient and low temperature), the use of spin trapping and spin scavenging (e.g. reaction with hydroxylamines), the detection of nitric oxide and the detection and quantification of some transition metal ions (particularly iron and copper) and their environment. MAJOR CONCLUSIONS: When used with care this technique can provide a wealth of valuable information on the presence of radicals and some transition metal ions in biological systems. It can provide definitive information on the identity of the species present and also information on their concentration, structure, mobility and interactions. It is however a technique that has major limitations and the user needs to understand the various pitfalls and shortcoming of the method to avoid making errors. GENERAL SIGNIFICANCE: EPR remains the most definitive method of identifying radicals in complex systems and is also a valuable method of examining radical kinetics, concentrations and structure. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
Authors: Reema Goel; Zachary Bitzer; Samantha M Reilly; Neil Trushin; Jonathan Foulds; Joshua Muscat; Jason Liao; Ryan J Elias; John P Richie Journal: Chem Res Toxicol Date: 2017-03-20 Impact factor: 3.739
Authors: Rhonda M Brand; Michael W Epperly; J Mark Stottlemyer; Erin M Skoda; Xiang Gao; Song Li; Saiful Huq; Peter Wipf; Valerian E Kagan; Joel S Greenberger; Louis D Falo Journal: J Invest Dermatol Date: 2016-10-26 Impact factor: 8.551
Authors: Tiffany A Moore; Iman M Ahmad; Kendra K Schmid; Ann M Berger; R Jeanne Ruiz; Rita H Pickler; Matthew C Zimmerman Journal: Biol Res Nurs Date: 2019-07-08 Impact factor: 2.522