Literature DB >> 23563535

Bio-nanocapsules for signal enhancement of alkaline phosphatase-linked immunosorbent assays.

Masumi Iijima1, Mikako Yamamoto, Nobuo Yoshimoto, Tomoaki Niimi, Shun'ichi Kuroda.   

Abstract

The bio-nanocapsules displaying about 240 molecules of immunoglobulin G Fc-binding Z domains (ZZ-BNCs) enhanced the signals of enzyme-linked immunosorbent assay by tethering the Fc regions of secondary antibodies (Abs), which were eliminated using high-molecular mass enzymes (e.g., alkaline phosphatase). By way of optimizing the distance between enzymes and Abs, ZZ-BNCs improved sensitivity independently of enzymes.

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Year:  2013        PMID: 23563535     DOI: 10.1271/bbb.120760

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN: 0916-8451            Impact factor:   2.043


  1 in total

1.  Cyclin B1 destruction box-mediated protein instability: the enhanced sensitivity of fluorescent-protein-based reporter gene system.

Authors:  Chao-Hsun Yang; Wan-Ting Kuo; Yun-Ting Chuang; Cheng-Yu Chen; Chih-Chien Lin
Journal:  Biomed Res Int       Date:  2013-12-15       Impact factor: 3.411

  1 in total

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