Literature DB >> 2356125

Enzymatic and NMR analysis of oligoribonucleotides synthesized with 2'-tert-butyldimethylsilyl protected cyanoethylphosphoramidite monomers.

Y Y Wang1, M H Lyttle, P N Borer.   

Abstract

The regioisomeric integrity of the internucleotide phosphate linkage in synthetic RNA using 2'-tert-butyldimethylsilyl protection was examined using enzymatic and NMR techniques. Two sets of DNA-RNA hybrid nonamers, T3XT5 and T5XT3 (where X = rA, rC, rG and U) and the tetramer AGCU were analyzed. Enzyme catalyzed hydrolysis of the nonamers with ribonuclease T2 showed that the linkage at the ribonucleotide was the desired 3'-5'. A control nonamer with a 2'-5' linkage was subjected to the enzyme, and showed no cleavage. High-resolution proton NMR of the tetramer also gave a favorable comparison with the same molecule obtained by non-chemical means.

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Year:  1990        PMID： 2356125      PMCID： PMC330943          DOI： 10.1093/nar/18.11.3347

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Authors:  E de Vroom; A Fidder; J E Marugg; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

2.  Stability of ribonucleic acid double-stranded helices.

Authors:  P N Borer; B Dengler; I Tinoco; O C Uhlenbeck
Journal:  J Mol Biol       Date:  1974-07-15       Impact factor: 5.469

3.  Improved estimation of secondary structure in ribonucleic acids.

Authors:  I Tinoco; P N Borer; B Dengler; M D Levin; O C Uhlenbeck; D M Crothers; J Bralla
Journal:  Nat New Biol       Date:  1973-11-14

4.  Solid-phase synthesis and high-resolution NMR studies of two synthetic double-helical RNA dodecamers: r(CGCGAAUUCGCG) and r(CGCGUAUACGCG).

Authors:  S H Chou; P Flynn; B Reid
Journal:  Biochemistry       Date:  1989-03-21       Impact factor: 3.162

5.  Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.

Authors:  T Tanaka; S Tamatsukuri; M Ikehara
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

6.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

7.  Selective 2'-benzoylation at the cis 2',3'-diols of protected ribonucleosides. New solid phase synthesis of RNA and DNA-RNA mixtures.

Authors:  T Kempe; F Chow; W I Sundquist; T J Nardi; B Paulson; S M Peterson
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

8.  Conformation and interaction of short nucleic acid double-stranded helices. I. Proton magnetic resonance studies on the nonexchangeable protons of ribosyl ApApGpCpUpU.

Authors:  P N Borer; L S Kan; P O Ts'o
Journal:  Biochemistry       Date:  1975-11-04       Impact factor: 3.162

9.  Conformation and interaction of short nucleic acid double-stranded helices. II. Proton magnetic resonance studies on the hydrogen-bonded NH-N protons of ribosyl ApApGpCpUpU helix.

Authors:  L S Kan; P N Borer; P O Ts'o
Journal:  Biochemistry       Date:  1975-11-04       Impact factor: 3.162

10.  Sequence and structure in double-stranded ribonucleic acid: (A-G-C-U)2 and (A-C-G-U)2.

Authors:  E Bubienko; M A Uniack; P N Borer
Journal:  Biochemistry       Date:  1981-11-24       Impact factor: 3.162
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  9 in total

1.  Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding.

Authors:  A E Walter; D H Turner; J Kim; M H Lyttle; P Müller; D H Mathews; M Zuker
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205

2.  RNA hairpin loop stability depends on closing base pair.

Authors:  M J Serra; M H Lyttle; T J Axenson; C A Schadt; D H Turner
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

3.  A new universal linker for solid phase DNA synthesis.

Authors:  M H Lyttle; D Hudson; R M Cook
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

4.  Structure of a small RNA hairpin.

Authors:  P W Davis; W Thurmes; I Tinoco
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

5.  Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 and HPLC purification of synthetic RNA.

Authors:  D Khare; J Orban
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

6.  Collisionally-induced dissociation of substituted pyrimidine antiviral agents: mechanisms of ion formation using gas phase hydrogen/deuterium exchange and electrospray ionization tandem mass spectrometry.

Authors:  Amin M Kamel; Burnaby Munson
Journal:  J Am Soc Mass Spectrom       Date:  2007-05-10       Impact factor: 3.109

7.  Negative ion formation in electrospray mass spectrometry.

Authors:  R F Straub; R D Voyksner
Journal:  J Am Soc Mass Spectrom       Date:  1993-07       Impact factor: 3.109

8.  The 'polysemous' codon--a codon with multiple amino acid assignment caused by dual specificity of tRNA identity.

Authors:  T Suzuki; T Ueda; K Watanabe
Journal:  EMBO J       Date:  1997-03-03       Impact factor: 11.598

9.  2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.

Authors:  Sebastian Motsch; Daniel Pfeffer; Clemens Richert
Journal:  Chembiochem       Date:  2020-03-04       Impact factor: 3.164
  9 in total

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