| Literature DB >> 23559216 |
Changbin Chen1, Ernest F Retzel.
Abstract
Improved transcriptome sequencing technologies (RNA-seq) have advanced our understanding of the tissue-specific transcriptome landscapes, including those of messenger RNAs, noncoding RNAs and small RNAs. However, transcriptome profiles of plant meiocytes remain challenging due to the lack of efficient methods to enrich meiocytes for the analysis of temporal and spatial gene expression patterns during meiosis. In this chapter, we describe a method to analyze the Arabidopsis meiotic transcriptome using isolated male meiocytes.Entities:
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Year: 2013 PMID： 23559216 DOI： 10.1007/978-1-62703-333-6_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745