OBJECTIVES/HYPOTHESIS: Tracheoplasty using costal cartilage grafts to enlarge the tracheal lumen was performed to treat congenital tracheal stenosis. Fibrotic granulomatous tissue was observed at the edge of grafted costal cartilage. We investigated the junction between the native hyaline cartilage and the engineered cartilage plates that were generated by auricular chondrocytes for fabricating the airway. STUDY DESIGN: Controlled, prospecive study. METHODS: In group 1, costal cartilage from New Zealand white rabbits was collected and implanted into a space created in the cervical trachea. In group 2, chondrocytes from auricular cartilages were seeded on absorbable scaffolds. These constructs were implanted in the subcutaneous space. Engineered cartilage plates were then implanted into the trachea after 3 weeks of implantation of the constructs. The grafts in group 1 and 2 were retrieved after 4 weeks. RESULTS: In group 1, histological studies of the junction between the native hyaline cartilage and the implanted costal cartilage demonstrated chondrogenic tissue in four anastomoses sides out of the 10 examined. In group 2, the junction between the native trachea and the engineered cartilage showed neocartilage tissue in nine anastomoses sides out of 10. CONCLUSIONS: Engineered cartilage may be beneficial for engineered airways, based on the findings of the junction between the native and engineered grafts.
OBJECTIVES/HYPOTHESIS: Tracheoplasty using costal cartilage grafts to enlarge the tracheal lumen was performed to treat congenital tracheal stenosis. Fibrotic granulomatous tissue was observed at the edge of grafted costal cartilage. We investigated the junction between the native hyaline cartilage and the engineered cartilage plates that were generated by auricular chondrocytes for fabricating the airway. STUDY DESIGN: Controlled, prospecive study. METHODS: In group 1, costal cartilage from New Zealand white rabbits was collected and implanted into a space created in the cervical trachea. In group 2, chondrocytes from auricular cartilages were seeded on absorbable scaffolds. These constructs were implanted in the subcutaneous space. Engineered cartilage plates were then implanted into the trachea after 3 weeks of implantation of the constructs. The grafts in group 1 and 2 were retrieved after 4 weeks. RESULTS: In group 1, histological studies of the junction between the native hyaline cartilage and the implanted costal cartilage demonstrated chondrogenic tissue in four anastomoses sides out of the 10 examined. In group 2, the junction between the native trachea and the engineered cartilage showed neocartilage tissue in nine anastomoses sides out of 10. CONCLUSIONS:Engineered cartilage may be beneficial for engineered airways, based on the findings of the junction between the native and engineered grafts.
Authors: Anna D Dikina; Daniel S Alt; Samuel Herberg; Alexandra McMillan; Hannah A Strobel; Zijie Zheng; Meng Cao; Bradley P Lai; Oju Jeon; Victoria Ivy Petsinger; Calvin U Cotton; Marsha W Rolle; Eben Alsberg Journal: Adv Sci (Weinh) Date: 2018-02-14 Impact factor: 16.806
Authors: Kuo-Hwa Wang; Richard Wan; Li-Hsuan Chiu; Yu-Hui Tsai; Chia-Lang Fang; John F Bowley; Kuan-Chou Chen; Hsin-Nung Shih; Wen-Fu Thomas Lai Journal: PLoS One Date: 2018-05-10 Impact factor: 3.240