Literature DB >> 23547013

DNA isolation from mammalian samples.

Ximeng Liu1, Shuko Harada.   

Abstract

DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.

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Year:  2013        PMID： 23547013     DOI： 10.1002/0471142727.mb0214s102

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  5 in total

1.  Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo.

Authors:  Brian Burkel; Brett A Morris; Suzanne M Ponik; Kristin M Riching; Kevin W Eliceiri; Patricia J Keely
Journal:  J Vis Exp       Date:  2016-05-09       Impact factor: 1.355

2.  A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing.

Authors:  Anthony N Snow; Aaron A Stence; Jonathan A Pruessner; Aaron D Bossler; Deqin Ma
Journal:  BMC Clin Pathol       Date:  2014-07-07

3.  HIV-1 CA Inhibitors Are Antagonized by Inositol Phosphate Stabilization of the Viral Capsid in Cells.

Authors:  Gregory A Sowd; Jiong Shi; Christopher Aiken
Journal:  J Virol       Date:  2021-10-06       Impact factor: 5.103

4.  The Combination of Single-Cell and Next-Generation Sequencing Can Reveal Mosaicism for BRCA2 Mutations and the Fine Molecular Details of Tumorigenesis.

Authors:  Alexandra Gráf; Márton Zsolt Enyedi; Lajos Pintér; Éva Kriston-Pál; Gábor Jaksa; Árpád Bálind; Éva Ezer; Péter Horváth; Farkas Sükösd; Ernő Kiss; Lajos Haracska
Journal:  Cancers (Basel)       Date:  2021-05-13       Impact factor: 6.639

5.  Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.

Authors:  Marina Gertsenstein; Lauryl M J Nutter
Journal:  Curr Protoc Mouse Biol       Date:  2018-03
  5 in total

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