OBJECTIVE: To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the TH1 cytokines interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) at the maternofetal interface. DESIGN: Case-control study. SETTING: University hospital unit of gynecology and obstetrics and research laboratories. PATIENT(S): Women undergoing suction curettage for spontaneous miscarriage (study group) and voluntary termination of pregnancy (control group) in the first trimester. INTERVENTION(S): Samples of decidua and villi collected and histologically examined at the time of suction curettage. MAIN OUTCOME MEASURE(S): Evaluation of all villous samples for karyotype with only euploid cases included; detection of transcripts of PRL, PRL-R, TNF-α, IFN-γ, and IL-2 by qualitative reverse-transcriptase-polymerase chain reaction (RT-PCR); investigation of pattern and site of expression by immunohistochemistry. RESULT(S): In both groups, PRL-R and IFN-γ were broadly expressed. The expression of PRL was impaired or absent in the villi of the study group compared with controls. Expression of TNF-α was reduced, although not statistically significantly, in both decidual and villous samples of the study group. Immunohistochemical analysis showed the lack of IL-2 expression in decidual specimens of the control group versus the full expression shown in the study group. CONCLUSION(S): Our results highlight the correspondence between PRL expression and vital pregnancy and the involvement of the TH1 cytokines with different specific roles at the implantation site. Prolactin and IL-2 may reciprocally influence expression.
OBJECTIVE: To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the TH1 cytokines interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) at the maternofetal interface. DESIGN: Case-control study. SETTING: University hospital unit of gynecology and obstetrics and research laboratories. PATIENT(S): Women undergoing suction curettage for spontaneous miscarriage (study group) and voluntary termination of pregnancy (control group) in the first trimester. INTERVENTION(S): Samples of decidua and villi collected and histologically examined at the time of suction curettage. MAIN OUTCOME MEASURE(S): Evaluation of all villous samples for karyotype with only euploid cases included; detection of transcripts of PRL, PRL-R, TNF-α, IFN-γ, and IL-2 by qualitative reverse-transcriptase-polymerase chain reaction (RT-PCR); investigation of pattern and site of expression by immunohistochemistry. RESULT(S): In both groups, PRL-R and IFN-γ were broadly expressed. The expression of PRL was impaired or absent in the villi of the study group compared with controls. Expression of TNF-α was reduced, although not statistically significantly, in both decidual and villous samples of the study group. Immunohistochemical analysis showed the lack of IL-2 expression in decidual specimens of the control group versus the full expression shown in the study group. CONCLUSION(S): Our results highlight the correspondence between PRL expression and vital pregnancy and the involvement of the TH1 cytokines with different specific roles at the implantation site. Prolactin and IL-2 may reciprocally influence expression.
Authors: Heather R Christensen; Michael K Murawsky; Nelson D Horseman; Tara A Willson; Karen A Gregerson Journal: Endocrinology Date: 2013-09-12 Impact factor: 4.736
Authors: J A Tamblyn; L E Jeffery; R Susarla; D M Lissauer; S L Coort; A Muñoz Garcia; K Knoblich; A L Fletcher; J N Bulmer; M D Kilby; M Hewison Journal: Reproduction Date: 2019-08 Impact factor: 3.906
Authors: A Olmos-Ortiz; M Déciga-García; E Preciado-Martínez; L Bermejo-Martínez; P Flores-Espinosa; I Mancilla-Herrera; C Irles; A C Helguera-Repetto; B Quesada-Reyna; V Goffin; L Díaz; V Zaga-Clavellina Journal: Mol Hum Reprod Date: 2019-10-28 Impact factor: 4.025