PURPOSE: Quantitative monitoring of the redox status is the foundation for redox-related treatment. The purpose of this study was to evaluate the reliability of a new depolarization curve method for plasma redox potential (ORP) monitoring. METHODS: Using the new method, we performed redox determinations for the first time under different sample-handling conditions, including redox titration experiments using KMnO4 and vitamin C and dynamic redox monitoring in burn patients. The relative ORP value (ΔORP) method (improved traditional method) was used as the reference. RESULTS: The new method's better reliability, electrochemical specificity and practicability, and known group validity, which are closely associated with the redox-related pathological processes of severe burns, were confirmed. Furthermore, bidirectional change in the redox status in severe burn patients was also observed for the first time. CONCLUSIONS: This simple, stable new method could be a better practical tool for making the dynamic monitoring of the redox status feasible and for providing useful quantitative information for the judgment of redox-related pathological process, thus improving corresponding individualized treatments that rely on quantitative adjustments to the redox status.
PURPOSE: Quantitative monitoring of the redox status is the foundation for redox-related treatment. The purpose of this study was to evaluate the reliability of a new depolarization curve method for plasma redox potential (ORP) monitoring. METHODS: Using the new method, we performed redox determinations for the first time under different sample-handling conditions, including redox titration experiments using KMnO4 and vitamin C and dynamic redox monitoring in burn patients. The relative ORP value (ΔORP) method (improved traditional method) was used as the reference. RESULTS: The new method's better reliability, electrochemical specificity and practicability, and known group validity, which are closely associated with the redox-related pathological processes of severe burns, were confirmed. Furthermore, bidirectional change in the redox status in severe burn patients was also observed for the first time. CONCLUSIONS: This simple, stable new method could be a better practical tool for making the dynamic monitoring of the redox status feasible and for providing useful quantitative information for the judgment of redox-related pathological process, thus improving corresponding individualized treatments that rely on quantitative adjustments to the redox status.
Authors: Anubhav Mittal; Friederike Göke; Richard Flint; Benjamin P T Loveday; Nichola Thompson; Brett Delahunt; Paul A Kilmartin; Garth J S Cooper; Julia MacDonald; Anthony Hickey; John A Windsor; Anthony R J Phillips Journal: Shock Date: 2010-05 Impact factor: 3.454
Authors: Leonard T Rael; Raphael Bar-Or; Rachel M Aumann; Denetta S Slone; Charles W Mains; David Bar-Or Journal: Biochem Biophys Res Commun Date: 2007-07-24 Impact factor: 3.575
Authors: Sander Rozemeijer; Angélique M E Spoelstra-de Man; Sophie Coenen; Bob Smit; Paul W G Elbers; Harm-Jan de Grooth; Armand R J Girbes; Heleen M Oudemans-van Straaten Journal: Nutrients Date: 2019-05-08 Impact factor: 5.717