BACKGROUND: Southeast Asia remains a critical region for the emergence of novel and/or zoonotic influenza, underscoring the importance of extensive sampling in rural areas where early transmission is most likely to occur. METHODS: In 2008, 800 adult participants from eight sites were enrolled in a prospective population-based study of avian influenza (AI) virus transmission where highly pathogenic avian influenza (HPAI) H5N1 virus had been reported in humans and poultry from 2006 to 2008. From their enrollment sera and questionnaires, we report risk factor findings for serologic evidence of previous infection with 18 AI virus strains. RESULTS: Serologic assays revealed no evidence of previous infection with 13 different low-pathogenic AI viruses or with HPAI avian-like A/Cambodia/R0404050/2007(H5N1). However, 21 participants had elevated antibodies against avian-like A/Hong Kong/1073/1999(H9N2), validated with a monoclonal antibody blocking ELISA assay specific for avian H9. CONCLUSIONS: Although cross-reaction from antibodies against human influenza viruses cannot be completely excluded, the study data suggest that a number of participants were previously infected with the avian-like A/Hong Kong/1073/1999(H9N2) virus, likely due to as yet unidentified environmental exposures. Prospective data from this cohort will help us better understand the serology of zoonotic influenza infection in a rural cohort in SE Asia.
BACKGROUND: Southeast Asia remains a critical region for the emergence of novel and/or zoonotic influenza, underscoring the importance of extensive sampling in rural areas where early transmission is most likely to occur. METHODS: In 2008, 800 adult participants from eight sites were enrolled in a prospective population-based study of avian influenza (AI) virus transmission where highly pathogenic avian influenza (HPAI) H5N1 virus had been reported in humans and poultry from 2006 to 2008. From their enrollment sera and questionnaires, we report risk factor findings for serologic evidence of previous infection with 18 AI virus strains. RESULTS: Serologic assays revealed no evidence of previous infection with 13 different low-pathogenic AI viruses or with HPAI avian-like A/Cambodia/R0404050/2007(H5N1). However, 21 participants had elevated antibodies against avian-like A/Hong Kong/1073/1999(H9N2), validated with a monoclonal antibody blocking ELISA assay specific for avian H9. CONCLUSIONS: Although cross-reaction from antibodies against human influenza viruses cannot be completely excluded, the study data suggest that a number of participants were previously infected with the avian-like A/Hong Kong/1073/1999(H9N2) virus, likely due to as yet unidentified environmental exposures. Prospective data from this cohort will help us better understand the serology of zoonotic influenza infection in a rural cohort in SE Asia.
Authors: Kendall P Myers; Christopher W Olsen; Sharon F Setterquist; Ana W Capuano; Kelley J Donham; Eileen L Thacker; James A Merchant; Gregory C Gray Journal: Clin Infect Dis Date: 2005-11-22 Impact factor: 9.079
Authors: T Rowe; R A Abernathy; J Hu-Primmer; W W Thompson; X Lu; W Lim; K Fukuda; N J Cox; J M Katz Journal: J Clin Microbiol Date: 1999-04 Impact factor: 5.948
Authors: Iain Stephenson; Karl G Nicholson; Reinhardt Glück; Robert Mischler; Robert W Newman; Abraham M Palache; Neville Q Verlander; Fiona Warburton; John M Wood; Maria C Zambon Journal: Lancet Date: 2003-12-13 Impact factor: 79.321
Authors: Kate A Harris; Gudrun S Freidl; Olga S Munoz; Sophie von Dobschuetz; Marco De Nardi; Barbara Wieland; Marion P G Koopmans; Katharina D C Stärk; Kristien van Reeth; Gwen Dauphin; Adam Meijer; Erwin de Bruin; Ilaria Capua; Andy A Hill; Rowena Kosmider; Jill Banks; Kim Stevens; Sylvie van der Werf; Vincent Enouf; Karen van der Meulen; Ian H Brown; Dennis J Alexander; Andrew C Breed Journal: Ecohealth Date: 2017-05-18 Impact factor: 3.184
Authors: Mokhtar R Gomaa; Ahmed S Kayed; Mona A Elabd; Dina Abu Zeid; Shaimaa A Zaki; Amira S El Rifay; Lobna S Sherif; Pamela P McKenzie; Robert G Webster; Richard J Webby; Mohamed A Ali; Ghazi Kayali Journal: J Infect Dis Date: 2014-10-29 Impact factor: 5.226
Authors: Adebimpe O Obadan; Brian J Kimble; Daniela Rajao; Kelly Lager; Jefferson J S Santos; Amy Vincent; Daniel R Perez Journal: J Gen Virol Date: 2015-05-18 Impact factor: 3.891