Literature DB >> 23536659

Guanine-nucleotide exchange factor RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase.

Ryan V Petty1, Ann C Palmenberg.   

Abstract

The leader (L) protein of encephalomyocarditis virus (EMCV) shuts off host cell nucleocytoplasmic trafficking (NCT) by inducing hyperphosphorylation of nuclear pore proteins. This dramatic effect by a nonenzymatic protein of 6 kDa is not well understood but clearly involves L binding to cellular Ran GTPase, a critical factor of active NCT. Exogenous GDP and GTP are inhibitory to L-Ran binding, but the guanine-nucleotide exchange factor RCC1 can relieve this inhibition. In the presence of RCC1, L binds Ran with a KD (equilibrium dissociation constant) of ≈ 3 nM and reaches saturation within 20 min. The results of fluorescently tagged nucleotide experiments suggest that L-Ran interactions affect the nucleotide-binding pocket of Ran.

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Year:  2013        PMID： 23536659      PMCID： PMC3648115          DOI： 10.1128/JVI.02493-12

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  13 in total

1.  The mengovirus leader protein suppresses alpha/beta interferon production by inhibition of the iron/ferritin-mediated activation of NF-kappa B.

Authors:  Jan Zoll; Willem J G Melchers; Jochem M D Galama; Frank J M van Kuppeveld
Journal:  J Virol       Date:  2002-10       Impact factor: 5.103

2.  The mengovirus leader protein blocks interferon-alpha/beta gene transcription and inhibits activation of interferon regulatory factor 3.

Authors:  Stanleyson V Hato; Celine Ricour; Barbara M Schulte; Kjerstin H W Lanke; Mike de Bruijni; Jan Zoll; Willem J G Melchers; Thomas Michiels; Frank J M van Kuppeveld
Journal:  Cell Microbiol       Date:  2007-12       Impact factor: 3.715

3.  Leader protein of encephalomyocarditis virus binds zinc, is phosphorylated during viral infection, and affects the efficiency of genome translation.

Authors:  C M Dvorak; D J Hall; M Hill; M Riddle; A Pranter; J Dillman; M Deibel; A C Palmenberg
Journal:  Virology       Date:  2001-11-25       Impact factor: 3.616

4.  Nucleocytoplasmic traffic disorder induced by cardioviruses.

Authors:  Peter V Lidsky; Stanleyson Hato; Maryana V Bardina; Alexei G Aminev; Ann C Palmenberg; Eugene V Sheval; Vladimir Y Polyakov; Frank J M van Kuppeveld; Vadim I Agol
Journal:  J Virol       Date:  2006-03       Impact factor: 5.103

5.  Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction.

Authors:  Y Cheng; W H Prusoff
Journal:  Biochem Pharmacol       Date:  1973-12-01       Impact factor: 5.858

6.  Nucleoporin phosphorylation triggered by the encephalomyocarditis virus leader protein is mediated by mitogen-activated protein kinases.

Authors:  Frederick W Porter; Bradley Brown; Ann C Palmenberg
Journal:  J Virol       Date:  2010-09-29       Impact factor: 5.103

7.  Characterization of Ran-driven cargo transport and the RanGTPase system by kinetic measurements and computer simulation.

Authors:  Dirk Görlich; Michael J Seewald; Katharina Ribbeck
Journal:  EMBO J       Date:  2003-03-03       Impact factor: 11.598

8.  Leader-induced phosphorylation of nucleoporins correlates with nuclear trafficking inhibition by cardioviruses.

Authors:  Frederick W Porter; Ann C Palmenberg
Journal:  J Virol       Date:  2008-12-10       Impact factor: 5.103

9.  The kinetic mechanism of Ran--nucleotide exchange catalyzed by RCC1.

Authors:  C Klebe; H Prinz; A Wittinghofer; R S Goody
Journal:  Biochemistry       Date:  1995-10-03       Impact factor: 3.162

10.  NMR structure of the mengovirus Leader protein zinc-finger domain.

Authors:  Claudia C Cornilescu; Frederick W Porter; Kate Qin Zhao; Ann C Palmenberg; John L Markley
Journal:  FEBS Lett       Date:  2008-02-20       Impact factor: 4.124
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  6 in total

1.  Solution structures of Mengovirus Leader protein, its phosphorylated derivatives, and in complex with nuclear transport regulatory protein, RanGTPase.

Authors:  Valjean R Bacot-Davis; Jessica J Ciomperlik; Holly A Basta; Claudia C Cornilescu; Ann C Palmenberg
Journal:  Proc Natl Acad Sci U S A       Date:  2014-10-20       Impact factor: 11.205

2.  Encephalomyocarditis virus leader is phosphorylated by CK2 and syk as a requirement for subsequent phosphorylation of cellular nucleoporins.

Authors:  Holly A Basta; Valjean R Bacot-Davis; Jessica J Ciomperlik; Ann C Palmenberg
Journal:  J Virol       Date:  2013-12-11       Impact factor: 5.103

3.  Encephalomyocarditis virus Leader protein hinge domain is responsible for interactions with Ran GTPase.

Authors:  Valjean R Bacot-Davis; Ann C Palmenberg
Journal:  Virology       Date:  2013-05-25       Impact factor: 3.616

4.  Binding interactions between the encephalomyocarditis virus leader and protein 2A.

Authors:  Ryan V Petty; Holly A Basta; Valjean R Bacot-Davis; Bradley A Brown; Ann C Palmenberg
Journal:  J Virol       Date:  2014-09-10       Impact factor: 5.103

5.  Cardiovirus Leader proteins bind exportins: Implications for virus replication and nucleocytoplasmic trafficking inhibition.

Authors:  Jessica J Ciomperlik; Holly A Basta; Ann C Palmenberg
Journal:  Virology       Date:  2015-10-19       Impact factor: 3.616

Review 6.  Nucleocytoplasmic Trafficking Perturbation Induced by Picornaviruses.

Authors:  Belén Lizcano-Perret; Thomas Michiels
Journal:  Viruses       Date:  2021-06-23       Impact factor: 5.048
  6 in total

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