BACKGROUND: DNA methylation at the five position of cytosine is well recognized as an important epigenetic modification in human health and disease. Recent evidences demonstrated that 5-methylcytosine (5-mC) by the TET family of enzymes can be converted to 5-hydroxymethylcytosine (5-hmC). Here, we use an ultrasensitive and accurate isotope-based LC-MS/MS method to precisely determine the levels of 5-hmC and 5-mC in colorectal cancer and the C-26 colon adenocarcinoma cell line. RESULTS: Our data showed that 5-hmC content is significantly reduced (approximately sixfold) in colorectal cancer as compared with adjacent normal tissue. Similarly, the ratio of 5-hmC to 5-mC dropped from 0.054 ± 0.005 in normal tissues, to 0.011 ± 0.002 in cancer. CONCLUSION: The analysis of 5-hmC levels and the ratio of 5-hmC:5-mC during tumor progression might provide insight into the role of this modification in cellular immortalization and transformation.
BACKGROUND: DNA methylation at the five position of cytosine is well recognized as an important epigenetic modification in human health and disease. Recent evidences demonstrated that 5-methylcytosine (5-mC) by the TET family of enzymes can be converted to 5-hydroxymethylcytosine (5-hmC). Here, we use an ultrasensitive and accurate isotope-based LC-MS/MS method to precisely determine the levels of 5-hmC and 5-mC in colorectal cancer and the C-26 colon adenocarcinoma cell line. RESULTS: Our data showed that 5-hmC content is significantly reduced (approximately sixfold) in colorectal cancer as compared with adjacent normal tissue. Similarly, the ratio of 5-hmC to 5-mC dropped from 0.054 ± 0.005 in normal tissues, to 0.011 ± 0.002 in cancer. CONCLUSION: The analysis of 5-hmC levels and the ratio of 5-hmC:5-mC during tumor progression might provide insight into the role of this modification in cellular immortalization and transformation.
Authors: Fengju Song; Christopher I Amos; Jeffrey E Lee; Christine G Lian; Shenying Fang; Hongliang Liu; Stuart MacGregor; Mark M Iles; Matthew H Law; Neal I Lindeman; Grant W Montgomery; David L Duffy; Anne E Cust; Mark A Jenkins; David C Whiteman; Richard F Kefford; Graham G Giles; Bruce K Armstrong; Joanne F Aitken; John L Hopper; Kevin M Brown; Nicholas G Martin; Graham J Mann; D Timothy Bishop; Julia A Newton Bishop; Peter Kraft; Abrar A Qureshi; Peter A Kanetsky; Nicholas K Hayward; David J Hunter; Qingyi Wei; Jiali Han Journal: Carcinogenesis Date: 2014-06-30 Impact factor: 4.944
Authors: Santiago Uribe-Lewis; Rory Stark; Thomas Carroll; Mark J Dunning; Martin Bachman; Yoko Ito; Lovorka Stojic; Silvia Halim; Sarah L Vowler; Andy G Lynch; Benjamin Delatte; Eric J de Bony; Laurence Colin; Matthieu Defrance; Felix Krueger; Ana-Luisa Silva; Rogier Ten Hoopen; Ashraf Ek Ibrahim; François Fuks; Adele Murrell Journal: Genome Biol Date: 2015-04-01 Impact factor: 13.583
Authors: Santiago Uribe-Lewis; Thomas Carroll; Suraj Menon; Anna Nicholson; Piotr J Manasterski; Douglas J Winton; Simon J A Buczacki; Adele Murrell Journal: Sci Rep Date: 2020-01-17 Impact factor: 4.379