Literature DB >> 235325

Enzymes of the human intestinal brush border membrane. Identification after gel electrophoretic separation.

D Maestracci, H Preiser, T Hedges, J Schmitz, R K Crane.   

Abstract

The postition of a number of human intestine brush border membrane enzyme activities in polyacrylamide gels after electrophoresis has been determined. These activities are, in order from the origin, maltase/glucoamylase, lactase/phlorizin hydrolase, maltase/sucrase/isomaltase, enteropeptidase, trehalase and gamma-glutamyl-transferase. Leucylnaphthylamide hydrolyzing activity was inactivated by sodium dodecylsulfate and its position was not determined. The positions of the activities have been correlated with the positions of protein bands previously determined. One such band situated between enteropeptidase and alkaline phosphatase has not been identified.

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Year:  1975        PMID： 235325     DOI： 10.1016/0005-2736(75)90173-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  12 in total

1.  Purification of rat intestinal maltase/glucoamylase and its anomalous dissociation either by heat or by low pH.

Authors:  P R Flanagan; G G Forstner
Journal:  Biochem J       Date:  1978-08-01       Impact factor: 3.857

2.  The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme.

Authors:  D A Grant; J Hermon-Taylor
Journal:  Biochem J       Date:  1976-05-01       Impact factor: 3.857

3.  Enzyme activity in partly dissociated fragments of rat intestinal maltase/glucoamylase.

Authors:  P R Flanagan; G G Forstner
Journal:  Biochem J       Date:  1979-02-01       Impact factor: 3.857

4.  The identification of rat intestinal membrane enzymes after electrophoresis on polyacrylamide gels containing sodium dodecyl sulphate.

Authors:  H P Hauri; J R Green
Journal:  Biochem J       Date:  1978-07-15       Impact factor: 3.857

5.  Adult onset sucrase-isomaltase deficiency with secondary disaccharidase deficiency resulting from severe dietary carbohydrate restriction.

Authors:  B T Cooper; J Scott; J Hopkins; T J Peters
Journal:  Dig Dis Sci       Date:  1983-05       Impact factor: 3.199

6.  Characterization of brush borders purified in iso-osmotic medium and microvillar membranes subfractionated from mouse small intestine.

Authors:  M Fujita; H Ohta; T Uezato
Journal:  Biochem J       Date:  1981-06-15       Impact factor: 3.857

7.  Synthesis of membrane glycoproteins in rat small-intestinal villus cells. Redistribution of L-[1,5,6-3H]fucose-labelled membrane glycoproteins among Golgi, lateral basal and microvillus membranes in vivo.

Authors:  A Quaroni; K Kirsch; M M Weiser
Journal:  Biochem J       Date:  1979-07-15       Impact factor: 3.857

8.  Surface-membrane biogenesis in rat intestinal epithelial cells at different stages of maturation.

Authors:  A Quaroni; K Kirsch; A Herscovics; K J Isselbacher
Journal:  Biochem J       Date:  1980-10-15       Impact factor: 3.857

9.  Selective release of inner core proteins from intestinal microvillus membrane by lithium diiodosalicylate.

Authors:  D Riendeau; J Lemaire; D Maestracci; L Lessard
Journal:  Mol Cell Biochem       Date:  1986-06       Impact factor: 3.396

10.  Species specificity of in vitro Escherichia coli adherence to host intestinal cell membranes and its correlation with in vivo colonization and infectivity.

Authors:  C P Cheney; P A Schad; S B Formal; E C Boedeker
Journal:  Infect Immun       Date:  1980-06       Impact factor: 3.441
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