OBJECTIVE: The identification of patients colonized or infected with carbapenemase-producing Enterobacteriaceae (CPE), in order to control and prevent the global spread of multidrug-resistant (MDR) pathogens. METHODS: From June 1 to June 15, 2012, eight Citrobacter freundii strains with reduced susceptibility to carbapenems were isolated from rectal swabs of hospitalized patients during active screening following the detection of a Klebsiella pneumoniae carbapenemase (KPC) -positive patient on the ward. All isolates were analyzed phenotypically and molecularly by PCR and sequencing. Genotype clustering was performed by multilocus sequence typing (MLST) analysis. RESULTS: The isolates showed high rates of multidrug resistance profile. A phenotypic assay for carbapenemase production suggested the presence of metallo-β-lactamase (MBL). The blaVIM-1 gene was detected in all imipenem-resistant C. freundii isolates. MLST showed that the C. freundii isolates shared the same sequence type (ST). Phylogenetic analysis revealed a strict relationship with an ST5C. freundii isolate from a diarrhea patient in China. CONCLUSIONS: Our findings showed that the active surveillance program for CPE was useful, not only for the detection of KPC-producers, but also to identify and control the spread of other MDR pathogens that could expand the spectrum of circulating MDR pathogens.
OBJECTIVE: The identification of patients colonized or infected with carbapenemase-producing Enterobacteriaceae (CPE), in order to control and prevent the global spread of multidrug-resistant (MDR) pathogens. METHODS: From June 1 to June 15, 2012, eight Citrobacter freundii strains with reduced susceptibility to carbapenems were isolated from rectal swabs of hospitalized patients during active screening following the detection of a Klebsiella pneumoniae carbapenemase (KPC) -positive patient on the ward. All isolates were analyzed phenotypically and molecularly by PCR and sequencing. Genotype clustering was performed by multilocus sequence typing (MLST) analysis. RESULTS: The isolates showed high rates of multidrug resistance profile. A phenotypic assay for carbapenemase production suggested the presence of metallo-β-lactamase (MBL). The blaVIM-1 gene was detected in all imipenem-resistant C. freundii isolates. MLST showed that the C. freundii isolates shared the same sequence type (ST). Phylogenetic analysis revealed a strict relationship with an ST5C. freundii isolate from a diarrheapatient in China. CONCLUSIONS: Our findings showed that the active surveillance program for CPE was useful, not only for the detection of KPC-producers, but also to identify and control the spread of other MDR pathogens that could expand the spectrum of circulating MDR pathogens.
Authors: A Baraniak; R Izdebski; J Fiett; M Herda; L P G Derde; M J M Bonten; A Adler; Y Carmeli; H Goossens; W Hryniewicz; C Brun-Buisson; M Gniadkowski Journal: Antimicrob Agents Chemother Date: 2015-12-28 Impact factor: 5.191
Authors: Yasufumi Matsumura; Gisele Peirano; Rebekah Devinney; Patricia A Bradford; Mary R Motyl; Mark D Adams; Liang Chen; Barry Kreiswirth; Johann D D Pitout Journal: J Antimicrob Chemother Date: 2017-08-01 Impact factor: 5.790
Authors: Olof H Sundin; Antonio Mendoza-Ladd; Mingtao Zeng; Diana Diaz-Arévalo; Elisa Morales; B Matthew Fagan; Javier Ordoñez; Philip Velez; Nishaal Antony; Richard W McCallum Journal: BMC Microbiol Date: 2017-07-17 Impact factor: 3.605
Authors: Ahmed Babiker; Daniel R Evans; Marissa P Griffith; Christi L McElheny; Mohamed Hassan; Lloyd G Clarke; Roberta T Mettus; Lee H Harrison; Yohei Doi; Ryan K Shields; Daria Van Tyne Journal: J Clin Microbiol Date: 2020-08-24 Impact factor: 5.948
Authors: Chengqian Qian; Yuhui Du; Huiying Li; Pan Wu; Lu Wang; Yi Wei; Hengchun Cao; Zhiqiu Yin; Yang Zhang; Yiming Zhu; Xi Guo; Bin Liu Journal: Future Microbiol Date: 2018-08-13 Impact factor: 3.165