Literature DB >> 23526490

Touring the Tomato: A Suite of Chemistry Laboratory Experiments.

Sayantani Sarkar1, Subhasish Chatterjee, Nancy Medina, Ruth E Stark.   

Abstract

An eight-session interdisciplinary laboratory curriculum has been designed using a suite of analytical chemistry techniques to study biomaterials derived from an inexpensive source such as the tomato fruit. A logical progression of research-inspired laboratory modules serves to "tour" the macroscopic characteristics of the fruit and the submicroscopic properties of its constituent cuticular biopolymers by atomic force microscopy (AFM), UV-visible, and nuclear magnetic resonance (NMR) methods at increasingly detailed molecular levels. The modular curriculum can be tailored for specialty undergraduate courses or summer high school workshops. By applying analytical tools to investigate biopolymers, making connections between molecular and microscale structure, and linking both structural regimes to the functional properties of natural polymers, groundwork is established for further student investigations at the interface of chemistry with biology or chemical engineering.

Entities:  

Keywords:  Bioanalytical Chemistry; Biophysical Chemistry; Curriculum; HPLC; Hands-On Learning/Manipulatives; High School/Introductory Chemistry; Laboratory Instruction; NMR Spectroscopy; Pedagogy; UV-Vis Spectroscopy; Upper-Division Undergraduate

Year:  2012        PMID: 23526490      PMCID: PMC3603577          DOI: 10.1021/ed3004148

Source DB:  PubMed          Journal:  J Chem Educ        ISSN: 0021-9584            Impact factor:   2.979


  6 in total

1.  The influence of water on the nanomechanical behavior of the plant biopolyester cutin as studied by AFM and solid-state NMR.

Authors:  A N Round; B Yan; S Dang; R Estephan; R E Stark; J D Batteas
Journal:  Biophys J       Date:  2000-11       Impact factor: 4.033

2.  Molecular characterization of the plant biopolyester cutin by AFM and spectroscopic techniques.

Authors:  José J Benítez; Antonio J Matas; Antonio Heredia
Journal:  J Struct Biol       Date:  2004-08       Impact factor: 2.867

3.  NMR characterization of hydration and thermal stress in tomato fruit cuticles.

Authors:  Ruth E Stark; Bin Yan; Suzanne M Stanley-Fernandez; Zhen-Jia Chen; Joel R Garbow
Journal:  Phytochemistry       Date:  2008-10-08       Impact factor: 4.072

Review 4.  Lycopene in tomatoes: chemical and physical properties affected by food processing.

Authors:  J Shi; M Le Maguer
Journal:  Crit Rev Biotechnol       Date:  2000       Impact factor: 8.429

5.  Tissue- and cell-type specific transcriptome profiling of expanding tomato fruit provides insights into metabolic and regulatory specialization and cuticle formation.

Authors:  Antonio J Matas; Trevor H Yeats; Gregory J Buda; Yi Zheng; Subhasish Chatterjee; Takayuki Tohge; Lalit Ponnala; Avital Adato; Asaph Aharoni; Ruth Stark; Alisdair R Fernie; Zhangjun Fei; James J Giovannoni; Jocelyn K C Rose
Journal:  Plant Cell       Date:  2011-11-01       Impact factor: 11.277

6.  Isolation and biophysical study of fruit cuticles.

Authors:  Subhasish Chatterjee; Sayantani Sarkar; Julia Oktawiec; Zhantong Mao; Olivia Niitsoo; Ruth E Stark
Journal:  J Vis Exp       Date:  2012-03-30       Impact factor: 1.355

  6 in total

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