OBJECTIVE: The aim of this study was to evaluate cases of oral epithelial dysplasia for biologically significant human papillomavirus (HPV) infection. STUDY DESIGN: Forty consecutive cases of high-grade dysplasia and 37 cases of low-grade dysplasia were examined for p16(INK4a) expression by immunohistochemistry. High-risk HPV infection was assessed in p16-positive cases using in situ hybridization. Proliferation index was assessed with MIB-1 immunohistochemistry. RESULTS: Eleven of 40 high-grade dysplasias and one of 37 low-grade dysplasias were p16 positive. High-risk HPV was detected in seven cases of p16-positive high-grade dysplasia. The difference between high- and low-grade dysplasia was statistically significant (P = .01). HPV-positive high-grade dysplasias showed a distinctive histologic appearance and MIB-1 labeling pattern. Most high-risk HPV-positive cases were seen in the floor of mouth. CONCLUSION: High-risk HPV was associated with a subset of cases of severe epithelial dysplasia/carcinoma in situ that demonstrated diffuse loss of squamous differentiation and a high proliferation index.
OBJECTIVE: The aim of this study was to evaluate cases of oral epithelial dysplasia for biologically significant human papillomavirus (HPV) infection. STUDY DESIGN: Forty consecutive cases of high-grade dysplasia and 37 cases of low-grade dysplasia were examined for p16(INK4a) expression by immunohistochemistry. High-risk HPV infection was assessed in p16-positive cases using in situ hybridization. Proliferation index was assessed with MIB-1 immunohistochemistry. RESULTS: Eleven of 40 high-grade dysplasias and one of 37 low-grade dysplasias were p16 positive. High-risk HPV was detected in seven cases of p16-positive high-grade dysplasia. The difference between high- and low-grade dysplasia was statistically significant (P = .01). HPV-positive high-grade dysplasias showed a distinctive histologic appearance and MIB-1 labeling pattern. Most high-risk HPV-positive cases were seen in the floor of mouth. CONCLUSION: High-risk HPV was associated with a subset of cases of severe epithelial dysplasia/carcinoma in situ that demonstrated diffuse loss of squamous differentiation and a high proliferation index.
Authors: Lisa M Rooper; Melina J Windon; Tahyna Hernandez; Brett Miles; Patrick K Ha; William R Ryan; Annemieke Van Zante; David W Eisele; Gypsyamber D'Souza; Carole Fakhry; William H Westra Journal: Am J Surg Pathol Date: 2020-05 Impact factor: 6.394
Authors: Rebecca D Chernock; Brian Nussenbaum; Wade L Thorstad; Yuling Luo; Xiao-Jun Ma; Samir K El-Mofty; James S Lewis Journal: Head Neck Pathol Date: 2013-10-23
Authors: Sujita Khanal; Brian S Shumway; Maryam Zahin; Rebecca A Redman; John D Strickley; Patrick J Trainor; Shesh N Rai; Shin-Je Ghim; Alfred Bennett Jenson; Joongho Joh Journal: Oncotarget Date: 2018-07-13