BACKGROUND: Aflatoxin contamination of various foodstuffs and agricultural commodities is a major problem worldwide. Several strategies have been reported for the detoxification of aflatoxins in contaminated foods and feeds, but all these methods have their own shortcomings. Traditional medicinal plants are potential sources of aflatoxin-detoxifying compounds. In this study a spray-dried formulation of Adhatoda vasica (L.) Nees leaf extract was prepared and its chemopreventive effect on aflatoxin B1 (AFB1)-induced biochemical changes in the liver and serum of Wistar rats was investigated. RESULTS: Administration of AFB1 (1.5 mg kg(-1) body weight (BW) intraperitoneally) to rats significantly reduced the activities of superoxide dismutase and catalase in liver tissues and increased the activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase and the levels of very-low-density lipoprotein, low-density lipoprotein and cholesterol in blood serum. However, pre-feeding of rats with A. vasica formulation (500 mg kg(-1) BW for 7 days) protected the animals from AFB1-induced biochemical changes during subsequent exposure to AFB1. CONCLUSION: Pre-feeding of rats with A. vasica formulation counteracted the hepatic dysfunction induced by subsequent treatment with AFB1. This formulated A. vasica extract offers a biologically safe alternative to detoxify aflatoxin and has huge potential to be used in the poultry industry to reduce aflatoxicosis.
BACKGROUND:Aflatoxin contamination of various foodstuffs and agricultural commodities is a major problem worldwide. Several strategies have been reported for the detoxification of aflatoxins in contaminated foods and feeds, but all these methods have their own shortcomings. Traditional medicinal plants are potential sources of aflatoxin-detoxifying compounds. In this study a spray-dried formulation of Adhatoda vasica (L.) Nees leaf extract was prepared and its chemopreventive effect on aflatoxin B1 (AFB1)-induced biochemical changes in the liver and serum of Wistar rats was investigated. RESULTS: Administration of AFB1 (1.5 mg kg(-1) body weight (BW) intraperitoneally) to rats significantly reduced the activities of superoxide dismutase and catalase in liver tissues and increased the activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase and the levels of very-low-density lipoprotein, low-density lipoprotein and cholesterol in blood serum. However, pre-feeding of rats with A. vasica formulation (500 mg kg(-1) BW for 7 days) protected the animals from AFB1-induced biochemical changes during subsequent exposure to AFB1. CONCLUSION: Pre-feeding of rats with A. vasica formulation counteracted the hepatic dysfunction induced by subsequent treatment with AFB1. This formulated A. vasica extract offers a biologically safe alternative to detoxify aflatoxin and has huge potential to be used in the poultry industry to reduce aflatoxicosis.
Authors: Donatus Wewura Adongo; Priscilla Kolibea Mante; Kennedy Kwami Edem Kukuia; Charles Kwaku Benneh; Robert Peter Biney; Eric Boakye-Gyasi; Nicholas Akinwale Titiloye; Eric Woode Journal: ScientificWorldJournal Date: 2018-05-20
Authors: Sadaf Naz; Sadia Alam; Waseem Ahmed; Shah Masaud Khan; Abdul Qayyum; Maimoona Sabir; Alia Naz; Asia Iqbal; Yamin Bibi; Sobia Nisa; Amany Salah Khalifa; Amal F Gharib; Ahmad El Askary Journal: Saudi J Biol Sci Date: 2021-10-16 Impact factor: 4.219