Literature DB >> 23515551

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

Karsten Becker1, Anders R Larsen, Robert L Skov, Gavin K Paterson, Mark A Holmes, Artur J Sabat, Alexander W Friedrich, Robin Köck, Georg Peters, André Kriegeskorte.   

Abstract

A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

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Year:  2013        PMID： 23515551      PMCID： PMC3716076          DOI： 10.1128/JCM.00075-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Prevalence of genes encoding for members of the staphylococcal leukotoxin family among clinical isolates of Staphylococcus aureus.

Authors:  Christof von Eiff; Alexander W Friedrich; Georg Peters; Karsten Becker
Journal:  Diagn Microbiol Infect Dis       Date:  2004-07       Impact factor: 2.803

2.  Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods?

Authors:  Karsten Becker; Isabelle Pagnier; Brigitte Schuhen; Frauke Wenzelburger; Alexander W Friedrich; Frank Kipp; Georg Peters; Christof von Eiff
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

3.  Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI.

Authors:  Duarte C Oliveira; Catarina Milheiriço; Hermínia de Lencastre
Journal:  Antimicrob Agents Chemother       Date:  2006-10       Impact factor: 5.191

4.  Failure of the BD GeneOhm StaphS/R assay for identification of Australian methicillin-resistant Staphylococcus aureus strains: duplex assays as the "gold standard" in settings of unknown SCCmec epidemiology.

Authors:  Lee Thomas; Sebastiaan van Hal; Matthew O'Sullivan; Pierre Kyme; Jon Iredell
Journal:  J Clin Microbiol       Date:  2008-10-22       Impact factor: 5.948

5.  Methicillin-resistant Staphylococcus aureus with the novel mecC gene variant isolated from a cat suffering from chronic conjunctivitis.

Authors:  Agathe Medhus; Jannice Schau Slettemeås; Lillian Marstein; Kjersti Wik Larssen; Marianne Sunde
Journal:  J Antimicrob Chemother       Date:  2012-12-04       Impact factor: 5.790

6.  Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.

Authors:  O G Brakstad; K Aasbakk; J A Maeland
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

7.  Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction.

Authors:  K Murakami; W Minamide; K Wada; E Nakamura; H Teraoka; S Watanabe
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

8.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

9.  eap Gene as novel target for specific identification of Staphylococcus aureus.

Authors:  Muzaffar Hussain; Christof von Eiff; Bhanu Sinha; Insa Joost; Mathias Herrmann; Georg Peters; Karsten Becker
Journal:  J Clin Microbiol       Date:  2007-12-19       Impact factor: 5.948

10.  Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus.

Authors:  Kunyan Zhang; Jo-Ann McClure; Sameer Elsayed; John M Conly
Journal:  Antimicrob Agents Chemother       Date:  2008-12-08       Impact factor: 5.191
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  11 in total

1.  A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens.

Authors:  Sabine Petersdorf; Miriam Herma; Meike Rosenblatt; Franziska Layer; Birgit Henrich
Journal:  J Clin Microbiol       Date:  2015-10-14       Impact factor: 5.948

2.  The mecA homolog mecC confers resistance against β-lactams in Staphylococcus aureus irrespective of the genetic strain background.

Authors:  Britta Ballhausen; André Kriegeskorte; Nina Schleimer; Georg Peters; Karsten Becker
Journal:  Antimicrob Agents Chemother       Date:  2014-04-21       Impact factor: 5.191

3.  Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.

Authors:  Karsten Becker; Olivier Denis; Sandrine Roisin; Alexander Mellmann; Evgeny A Idelevich; Dennis Knaack; Sarah van Alen; André Kriegeskorte; Robin Köck; Frieder Schaumburg; Georg Peters; Britta Ballhausen
Journal:  J Clin Microbiol       Date:  2015-10-21       Impact factor: 5.948

4.  Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.

Authors:  Verónica K C Pérez; Dircéia A C Custódio; Eduarda M M Silva; Julia de Oliveira; Alessandro S Guimarães; Maria A V P Brito; Antônio F Souza-Filho; Marcos B Heinemann; Andrey P Lage; Elaine M S Dorneles
Journal:  Braz J Microbiol       Date:  2020-08-20       Impact factor: 2.476

5.  Comparison of Different Phenotypic Approaches To Screen and Detect mecC-Harboring Methicillin-Resistant Staphylococcus aureus.

Authors:  André Kriegeskorte; Evgeny A Idelevich; Andreas Schlattmann; Franziska Layer; Birgit Strommenger; Olivier Denis; Gavin K Paterson; Mark A Holmes; Guido Werner; Karsten Becker
Journal:  J Clin Microbiol       Date:  2017-12-26       Impact factor: 5.948

Review 6.  Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology.

Authors:  Sahreena Lakhundi; Kunyan Zhang
Journal:  Clin Microbiol Rev       Date:  2018-09-12       Impact factor: 26.132

Review 7.  The emergence of mecC methicillin-resistant Staphylococcus aureus.

Authors:  Gavin K Paterson; Ewan M Harrison; Mark A Holmes
Journal:  Trends Microbiol       Date:  2013-12-09       Impact factor: 17.079

8.  A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.

Authors:  Maria W Smith; Andrei L Ghindilis; Ihab A Seoudi; Kenneth Smith; Rosalind Billharz; Holly M Simon
Journal:  PLoS One       Date:  2014-05-15       Impact factor: 3.240

9.  PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.

Authors:  Ying Liu; Jiang Zhang; Yinduo Ji
Journal:  Open Microbiol J       Date:  2016-04-14

10.  Periorbital infections and conjunctivitis due to Panton-Valentine Leukocidin (PVL) positive Staphylococcus aureus in children.

Authors:  Pia-Alice Hoppe; Leif G Hanitsch; Rasmus Leistner; Michaela Niebank; Christoph Bührer; Horst von Bernuth; Renate Krüger
Journal:  BMC Infect Dis       Date:  2018-08-06       Impact factor: 3.090
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