OBJECTIVE: The scavenger receptor CD36 facilitates the cellular uptake of long-chain fatty acids. As CD36-deficiency attenuates the development of high fat diet (HFD)-induced obesity, the role of CD36-deficiency in preadipocyte recruitment and adipocyte function was set out to characterize. DESIGN AND METHODS: Fat cell size and number were determined in gonadal, visceral, and subcutaneous adipose tissue of CD36(-/-) and WT mice after 6 weeks on HFD. Basal lipolysis and insulin-inhibited lipolysis were investigated in gonadal adipose tissue. RESULTS: CD36(-/-) mice showed a reduction in adipocyte size in all fat pads. Gonadal adipose tissue also showed a lower total number of adipocytes because of a lower number of very small adipocytes (diameter <50 μm). This was accompanied by an increased pool of preadipocytes, which suggests that CD36-deficiency reduces the capacity of preadipocytes to become adipocytes. Regarding lipolysis, in adipose tissue from CD36(-/-) mice, cAMP levels were increased and both basal and 8-bromo-cAMP stimulated lipolysis were higher. However, insulin-mediated inhibition of lipolysis was more potent in CD36(-/-) mice. CONCLUSIONS: These results indicate that during fat depot expansion, CD36-deficiency negatively affects preadipocyte recruitment and that in mature adipocytes, CD36-deficiency is associated with increased basal lipolysis and insulin responsiveness.
OBJECTIVE: The scavenger receptor CD36 facilitates the cellular uptake of long-chain fatty acids. As CD36-deficiency attenuates the development of high fat diet (HFD)-induced obesity, the role of CD36-deficiency in preadipocyte recruitment and adipocyte function was set out to characterize. DESIGN AND METHODS: Fat cell size and number were determined in gonadal, visceral, and subcutaneous adipose tissue of CD36(-/-) and WT mice after 6 weeks on HFD. Basal lipolysis and insulin-inhibited lipolysis were investigated in gonadal adipose tissue. RESULTS:CD36(-/-) mice showed a reduction in adipocyte size in all fat pads. Gonadal adipose tissue also showed a lower total number of adipocytes because of a lower number of very small adipocytes (diameter <50 μm). This was accompanied by an increased pool of preadipocytes, which suggests that CD36-deficiency reduces the capacity of preadipocytes to become adipocytes. Regarding lipolysis, in adipose tissue from CD36(-/-) mice, cAMP levels were increased and both basal and 8-bromo-cAMP stimulated lipolysis were higher. However, insulin-mediated inhibition of lipolysis was more potent in CD36(-/-) mice. CONCLUSIONS: These results indicate that during fat depot expansion, CD36-deficiency negatively affects preadipocyte recruitment and that in mature adipocytes, CD36-deficiency is associated with increased basal lipolysis and insulin responsiveness.
Authors: José K van den Heuvel; Mariëtte R Boon; Ingmar van Hengel; Emma Peschier-van der Put; Lianne van Beek; Vanessa van Harmelen; Ko Willems van Dijk; Alberto M Pereira; Hazel Hunt; Joseph K Belanoff; Patrick C N Rensen; Onno C Meijer Journal: Br J Pharmacol Date: 2016-04-24 Impact factor: 8.739
Authors: Lianne van Beek; Jan B van Klinken; Amanda C M Pronk; Andrea D van Dam; Eline Dirven; Patrick C N Rensen; Frits Koning; Ko Willems van Dijk; Vanessa van Harmelen Journal: Diabetologia Date: 2015-05-12 Impact factor: 10.122
Authors: María E Cabalén; María F Cabral; Liliana M Sanmarco; Marta C Andrada; Luisina I Onofrio; Nicolás E Ponce; María P Aoki; Susana Gea; Roxana C Cano Journal: Oncotarget Date: 2016-03-22
Authors: Christopher A Lavery; Mariola Kurowska-Stolarska; William M Holmes; Iona Donnelly; Muriel Caslake; Andrew Collier; Andrew H Baker; Ashley M Miller Journal: Obesity (Silver Spring) Date: 2016-07-05 Impact factor: 5.002