| Literature DB >> 23506236 |
Sijie Chen1, Yuning Hong, Yang Liu, Jianzhao Liu, Chris W T Leung, Min Li, Ryan T K Kwok, Engui Zhao, Jacky W Y Lam, Yong Yu, Ben Zhong Tang.
Abstract
Intracellular pH (pHi) is an important parameter associated with cellular behaviors and pathological conditions. Sensing pHi and monitoring its changes in live cells are essential but challenging due to the lack of effective probes. We herein report a pH-sensitive fluorogen for pHi sensing and tracking. The dye is a tetraphenylethene-cyanine adduct (TPE-Cy). It is biocompatible and cell-permeable. Upon diffusing into cells, it responds sensitively to pHi in the entire physiological range, visualizing the acidic and basic compartments with intense red and blue emissions, respectively. The ratiometric signal of the red and blue channels can thus serve as an indicator for local proton concentration. The utility of TPE-Cy in pHi imaging and monitoring is demonstrated with the use of confocal microscopy, ratiometric analysis, and flow cytometry.Entities:
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Year: 2013 PMID： 23506236 DOI： 10.1021/ja400337p
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419