Literature DB >> 23504430

Droplets as reaction compartments for protein nanotechnology.

Sean R A Devenish1, Miriam Kaltenbach, Martin Fischlechner, Florian Hollfelder.   

Abstract

Extreme miniaturization of biological and chemical reactions in pico- to nanoliter microdroplets is emerging as an experimental paradigm that enables more experiments to be carried out with much lower sample consumption, paving the way for high-throughput experiments. This review provides the protein scientist with an experimental framework for (a) formation of polydisperse droplets by emulsification or, alternatively, of monodisperse droplets using microfluidic devices; (b) construction of experimental rigs and microfluidic chips for this purpose; and (c) handling and analysis of droplets.

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Year:  2013        PMID： 23504430     DOI： 10.1007/978-1-62703-354-1_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Evolution of enzyme catalysts caged in biomimetic gel-shell beads.

Authors:  Martin Fischlechner; Yolanda Schaerli; Mark F Mohamed; Santosh Patil; Chris Abell; Florian Hollfelder
Journal:  Nat Chem       Date:  2014-07-20       Impact factor: 24.427

2.  Towards the directed evolution of protein materials.

Authors:  Anton Kan; Neel S Joshi
Journal:  MRS Commun       Date:  2019-04-08       Impact factor: 2.566

3.  Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.

Authors:  Liisa D van Vliet; Pierre-Yves Colin; Florian Hollfelder
Journal:  Interface Focus       Date:  2015-08-06       Impact factor: 3.906

4.  Bacterial Microcolonies in Gel Beads for High-throughput Screening.

Authors:  Yolanda Schaerli
Journal:  Bio Protoc       Date:  2018-07-05

5.  A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth.

Authors:  WeiYue Chen; Edward Avezov; Simon C Schlachter; Fabrice Gielen; Romain F Laine; Heather P Harding; Florian Hollfelder; David Ron; Clemens F Kaminski
Journal:  Biophys J       Date:  2015-03-10       Impact factor: 4.033

6.  In vitro affinity screening of protein and peptide binders by megavalent bead surface display.

Authors:  Letizia Diamante; Pietro Gatti-Lafranconi; Yolanda Schaerli; Florian Hollfelder
Journal:  Protein Eng Des Sel       Date:  2013-08-26       Impact factor: 1.650

7.  A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition.

Authors:  Fabrice Gielen; Liisa van Vliet; Bartosz T Koprowski; Sean R A Devenish; Martin Fischlechner; Joshua B Edel; Xize Niu; Andrew J deMello; Florian Hollfelder
Journal:  Anal Chem       Date:  2013-04-24       Impact factor: 6.986

Review 8.  Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

Authors:  Andrew Currin; Neil Swainston; Philip J Day; Douglas B Kell
Journal:  Chem Soc Rev       Date:  2015-03-07       Impact factor: 54.564

9.  A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.

Authors:  Che Fai Alex Wong; Liisa van Vliet; Swapnil Vilas Bhujbal; Chengzhi Guo; Marit Sletmoen; Bjørn Torger Stokke; Florian Hollfelder; Rahmi Lale
Journal:  ACS Synth Biol       Date:  2021-07-14       Impact factor: 5.110
  9 in total

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