| Literature DB >> 23504369 |
Abstract
Small interfering RNA (siRNA) therapeutics have advanced from bench to clinical trials in recent years, along with new tools developed to enable detection of siRNA delivered at the organ, cell, and subcellular levels. Preclinical models of siRNA delivery have benefitted from methodologies such as stem-loop quantitative polymerase chain reaction, histological in situ immunofluorescent staining, endosomal escape assay, and RNA-induced silencing complex loading assay. These technologies have accelerated the detection and optimization of siRNA platforms to overcome the challenges associated with delivering therapeutic oligonucleotides to the cytosol of specific target cells. This review focuses on the methodologies and their application in the biodistribution of siRNA delivered by lipid nanoparticles.Entities:
Keywords: LNP; QWBA; RISC; biodistribution; delivery; endosomal escape; immunofluorescence staining; intravital imaging; lipid nanoparticle; siRNA; stem-loop PCR
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Year: 2013 PMID： 23504369 PMCID： PMC3715328 DOI： 10.1369/0022155413484152
Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479