BACKGROUND: A common Human Leukocyte Antigen (HLA) class II allele, DQβ1*03:01, seems to be associated with Bullous pemphigoid (BP) in Caucasians whereas previous studies in other ethnic groups showed other HLA class II alleles as genetic predisposing factors for BP. OBJECTIVE: To investigate the association of HLA class II alleles and haplotypes with BP in Iranian population. METHODS: Fifty patients with Bullous pemphigoid and 180 geographically matched, healthy individuals as control group enrolled into this study. HLA typing of class II (DR and DQ alleles) was carried out using polymerase chain reaction based on sequence-specific primers method. RESULTS: Class II DQA1 and DQB1 typing showed a significantly higher frequency of HLA-DQA1*05:01 (45% vs. 33%, p=0.03), HLA-DQB1*03:01 (36% vs. 23.6%, p=0.02) and HLA-DQB1*04:01 (4% vs. 1.6%, p=0.04) in the BP patients compared with controls. For DRB1 allele frequencies, there were no significant disease associations. The frequency of DRB1*08:01/DQA1*05:01/DQB1*03:01 (3% vs. 0%, p=0.02) haplotype showed an increase among patients compared with controls. CONCLUSION: Our data suggest that Iranian patients with BP present the same genetic predisposition linked to HLA-DQB1*03:01 previously reported in Caucasians.
BACKGROUND: A common Human Leukocyte Antigen (HLA) class II allele, DQβ1*03:01, seems to be associated with Bullous pemphigoid (BP) in Caucasians whereas previous studies in other ethnic groups showed other HLA class II alleles as genetic predisposing factors for BP. OBJECTIVE: To investigate the association of HLA class II alleles and haplotypes with BP in Iranian population. METHODS: Fifty patients with Bullous pemphigoid and 180 geographically matched, healthy individuals as control group enrolled into this study. HLA typing of class II (DR and DQ alleles) was carried out using polymerase chain reaction based on sequence-specific primers method. RESULTS:Class II DQA1 and DQB1 typing showed a significantly higher frequency of HLA-DQA1*05:01 (45% vs. 33%, p=0.03), HLA-DQB1*03:01 (36% vs. 23.6%, p=0.02) and HLA-DQB1*04:01 (4% vs. 1.6%, p=0.04) in the BP patients compared with controls. For DRB1 allele frequencies, there were no significant disease associations. The frequency of DRB1*08:01/DQA1*05:01/DQB1*03:01 (3% vs. 0%, p=0.02) haplotype showed an increase among patients compared with controls. CONCLUSION: Our data suggest that Iranian patients with BP present the same genetic predisposition linked to HLA-DQB1*03:01 previously reported in Caucasians.
Authors: Enno Schmidt; Saleh M Ibrahim; Christian Schwarm; Damian Gola; Maike M Holtsche; Anabelle Dieterich; Anita Bhandari; Miriam Freitag; Peter Nürnberg; Mohammad Toliat; Wolfgang Lieb; Michael Wittig; André Franke; Margitta Worm; Michael Sticherling; Jan Ehrchen; Claudia Günther; Regine Gläser; Wiebke K Peitsch; Miklós Sárdy; Rüdiger Eming; Michael Hertl; Sandrine Benoit; Matthias Goebeler; Claudia Pföhler; Manfred Kunz; Alexander Kreuter; Nina van Beek; Jeanette Erdmann; Hauke Busch; Detlef Zillikens; Christian D Sadik; Misa Hirose; Inke R König Journal: Orphanet J Rare Dis Date: 2021-05-19 Impact factor: 4.123